Overview

  • Product name
  • Description
    Rabbit polyclonal to Gli1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Human, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Mouse Gli1 aa 600-700 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P47806

  • Positive control
    • This antibody gave a positive signal in the following Mouse tissue lysates: E14 Embryonic Brain; E16 Embryonic Brain; E18 Embryonic Brain.

Properties

Applications

Our Abpromise guarantee covers the use of ab167388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).Can be blocked with Mouse Gli1 peptide (ab201523).

Target

  • Function
    Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation.
  • Tissue specificity
    Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract.
  • Sequence similarities
    Belongs to the GLI C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated in vitro by ULK3.
  • Cellular localization
    Cytoplasm. Nucleus. Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gli 1 antibody
    • GLI antibody
    • GLI family zinc finger 1 antibody
    • GLI Kruppel family member 1 antibody
    • gli1 antibody
    • GLI1_HUMAN antibody
    • Glioma associated oncogene 1 antibody
    • Glioma associated oncogene homolog 1 (zinc finger protein) antibody
    • Glioma associated oncogene homolog antibody
    • Glioma-associated oncogene antibody
    • Oncogene GLI antibody
    • Zfp 5 antibody
    • Zfp5 antibody
    • Zinc finger protein GLI 1 antibody
    • Zinc finger protein GLI1 antibody
    see all

Images

  • All lanes : Anti-Gli1 antibody (ab167388) at 1 µg/ml

    Lane 1 : E14 Mouse Embryo Brain Tissue Lysate
    Lane 2 : E16 Ms Embryo Brain Tissue Lysate
    Lane 3 : E18 Ms Embryo Brain Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 118 kDa
    Observed band size: 118 kDa
    Additional bands at: 45 kDa (possible non-specific binding)


    Exposure time: 20 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab167388 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

References

ab167388 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - nuclear (fibroblasts)
Specification
fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Dr. Adeline Vulin-Chaffiol

Verified customer

Submitted Mar 25 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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