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  1. Link

    gli1-antibody-ab167388.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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Anti-Gli1 antibody (ab167388)

  • Datasheet
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Western blot - Anti-Gli1 antibody (ab167388)

    Key features and details

    • Rabbit polyclonal to Gli1
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

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    Overview

    • Product name

      Anti-Gli1 antibody
      See all Gli1 primary antibodies
    • Description

      Rabbit polyclonal to Gli1
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse
      Predicted to work with: Rat, Rabbit, Human, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
    • Immunogen

      Synthetic peptide corresponding to Mouse Gli1 aa 600-700 conjugated to keyhole limpet haemocyanin.
      Database link: P47806
      (Peptide available as ab201523)

    • Positive control

      • This antibody gave a positive signal in the following Mouse tissue lysates: E14 Embryonic Brain; E16 Embryonic Brain; E18 Embryonic Brain.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Zinc Finger
      • Neuroscience
      • Neurology process
      • Neural Signal Transduction
      • Stem Cells
      • Signaling Pathways
      • Hedgehog
      • Nuclear
      • Cancer
      • Oncoproteins/suppressors
      • Tumor suppressors
      • PTC & Wnt pathway
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Krueppel like factor

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Immunizing Peptide (Blocking)

      • Mouse Gli1 peptide (ab201523)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Gli1 protein (ab152427)

    Applications

    Our Abpromise guarantee covers the use of ab167388 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use a concentration of 1 µg/ml. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).Can be blocked with Mouse Gli1 peptide (ab201523).

    Target

    • Function

      Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation.
    • Tissue specificity

      Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract.
    • Sequence similarities

      Belongs to the GLI C2H2-type zinc-finger protein family.
      Contains 5 C2H2-type zinc fingers.
    • Post-translational
      modifications

      Phosphorylated in vitro by ULK3.
    • Cellular localization

      Cytoplasm. Nucleus. Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1.
    • Target information above from: UniProt accession P08151 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 2735 Human
      • Entrez Gene: 14632 Mouse
      • Entrez Gene: 140589 Rat
      • Omim: 165220 Human
      • SwissProt: P08151 Human
      • SwissProt: P47806 Mouse
      • Unigene: 632702 Human
      • Unigene: 391450 Mouse
      • Unigene: 219157 Rat
      see all
    • Alternative names

      • Gli 1 antibody
      • GLI antibody
      • GLI family zinc finger 1 antibody
      • GLI Kruppel family member 1 antibody
      • gli1 antibody
      • GLI1_HUMAN antibody
      • Glioma associated oncogene 1 antibody
      • Glioma associated oncogene homolog 1 (zinc finger protein) antibody
      • Glioma associated oncogene homolog antibody
      • Glioma-associated oncogene antibody
      • Oncogene GLI antibody
      • Zfp 5 antibody
      • Zfp5 antibody
      • Zinc finger protein GLI 1 antibody
      • Zinc finger protein GLI1 antibody
      see all

    Images

    • Western blot - Anti-Gli1 antibody (ab167388)
      Western blot - Anti-Gli1 antibody (ab167388)
      All lanes : Anti-Gli1 antibody (ab167388) at 1 µg/ml

      Lane 1 : E14 Mouse Embryo Brain Tissue Lysate
      Lane 2 : E16 Ms Embryo Brain Tissue Lysate
      Lane 3 : E18 Ms Embryo Brain Tissue Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 118 kDa
      Observed band size: 118 kDa
      Additional bands at: 45 kDa (possible non-specific binding)


      Exposure time: 20 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab167388 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab167388? Please let us know so that we can cite the reference in this datasheet.

    ab167388 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Western blot abreview for Anti-Gli1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - nuclear (fibroblasts)
    Specification
    fibroblasts
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Dr. Adeline Vulin-Chaffiol

    Verified customer

    Submitted Mar 25 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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