Key features and details
- Rabbit polyclonal to Gli1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Gli1 antibody
See all Gli1 primary antibodies
DescriptionRabbit polyclonal to Gli1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Gli1 aa 768-1106 (C terminal).
QQASYPDPTQETW GEFPSHSGLY PGPKALGGTY SQCPRLEHYG QVQVKPEQGC PVGSDSTGLA PCLNAHPSEG PPHPQPLFSH YPQPSPPQYL QSGPYTQPPP DYLPSEPRPC LDFDSPTHST GQLKAQLVCN YVQSQQELLW EGGGREDAPA QEPSYQSPKF LGGSQVSPSR AKAPVNTYGP GFGPNLPNHK SGSYPTPSPC HENFVVGANR ASHRAAAPPR LLPPLPTCYG PLKVGGTNPS CGHPEVGRLG GGPALYPPPE GQVCNPLDSL DLDNTQLDFV AILDEPQGLS PPPSHDQRGS SGHTPPPSGP PNMAVGNMSV LLRSLPGETE FLNSSA
Database link: P08151
- WB: Recombinant human Gli1 protein; U87, MCF7, HeLa, SKOV, HT1080, COLO320 and HepG2 whole cell lysate (ab7900). IHC-P: Human intestinal cancer tissue.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.025% Sodium azide
Constituents: 2.5% BSA, 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab217326 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).
The detection limit for GLi1 is approximately 0.25 ng/lane under reducing conditions.
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionActs as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation.
Tissue specificityTestis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract.
Sequence similaritiesBelongs to the GLI C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers.
modificationsPhosphorylated in vitro by ULK3.
Cellular localizationCytoplasm. Nucleus. Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1.
- Information by UniProt
- Gli 1 antibody
- GLI antibody
- GLI family zinc finger 1 antibody
All lanes : Anti-Gli1 antibody (ab217326) at 0.5 µg/ml
Lane 1 : U87 whole cell lysate
Lane 2 : MCF7 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : SKOV whole cell lysate
Lane 5 : HT1080 whole cell lysate
Lane 6 : COLO320 whole cell lysate
Lane 7 : HepG2 whole cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 118 kDa
Immunohistochemical analysis of paraffin embedded mouse testis tissue staining Gli1 with ab217326 at 1µg/ml. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1/100 dilution. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. DAPI was used as a nucelar counterstain (blue).
Immunohistochemical analysis of paraffin-embedded human intestinal cancer tissue labeling Gil1 with ab217326 at 1 µg/mL.
Anti-Gli1 antibody (ab217326) at 0.5 µg/ml + recombinant human GLI1 protein, 5 ng
Predicted band size: 118 kDa
ab217326 has been referenced in 1 publication.
- Zhao Z et al. Degalactotigonin, a Natural Compound from Solanum nigrum L., Inhibits Growth and Metastasis of Osteosarcoma through GSK3ß Inactivation-Mediated Repression of the Hedgehog/Gli1 Pathway. Clin Cancer Res 24:130-144 (2018). PubMed: 28951519