Product nameAnti-Gli1 antibody [EPR4523]
See all Gli1 primary antibodies
DescriptionRabbit monoclonal [EPR4523] to Gli1
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human Gli1 aa 1-100. The exact sequence is proprietary.
- RMS13 and A673 cell lysates; Human brain and testis tissues; A673 cells.
This antibody was developed as part of a collaboration with the lab of Martin Fernandez-Zapico at the Mayo Clinic.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab134906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Predicted molecular weight: 118 kDa.
FunctionActs as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation.
Tissue specificityTestis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract.
Sequence similaritiesBelongs to the GLI C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers.
modificationsPhosphorylated in vitro by ULK3.
Cellular localizationCytoplasm. Nucleus. Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1.
- Information by UniProt
- Gli 1 antibody
- GLI antibody
- GLI family zinc finger 1 antibody
Anti-Gli1 antibody [EPR4523] (ab134906) at 1/1000 dilution (purified) + RMS13 cell lysate at 10 µg
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 118 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes : Anti-Gli1 antibody [EPR4523] (ab134906) at 1/1000 dilution (unpurified)
Lane 1 : RMS13 cell lysate
Lane 2 : A673 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution
Predicted band size: 118 kDa
This product has been referenced in:
- Liu Y et al. Dihydroartemisinin induces apoptosis and inhibits proliferation, migration, and invasion in epithelial ovarian cancer via inhibition of the hedgehog signaling pathway. Cancer Med 7:5704-5715 (2018). Read more (PubMed: 30338663) »
- Teixeira JD et al. Sonic Hedgehog signaling and Gli-1 during embryonic chick myogenesis. Biochem Biophys Res Commun 507:496-502 (2018). Read more (PubMed: 30449599) »