Recombinant Anti-Gli1 antibody [EPR4523] - BSA and Azide free (ab240088)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4523] to Gli1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Gli1 antibody [EPR4523] - BSA and Azide free
See all Gli1 primary antibodies -
Description
Rabbit monoclonal [EPR4523] to Gli1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab240088 is the carrier-free version of ab134906.
This antibody was developed as part of a collaboration with the lab of Martin Fernandez-Zapico at the Mayo Clinic.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4523 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240088 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 118 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 118 kDa. |
Target
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Function
Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation. -
Tissue specificity
Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. -
Sequence similarities
Belongs to the GLI C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. -
Post-translational
modificationsPhosphorylated in vitro by ULK3. -
Cellular localization
Cytoplasm. Nucleus. Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1. - Information by UniProt
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Database links
- Entrez Gene: 2735 Human
- Omim: 165220 Human
- SwissProt: P08151 Human
- Unigene: 632702 Human
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Alternative names
- Gli 1 antibody
- GLI antibody
- GLI family zinc finger 1 antibody
see all
Images
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All lanes : Anti-Gli1 antibody [EPR4523] (ab134906) at 1/1000 dilution
Lane 1 : RMS 13 (human rhabdomyosarcoma fibroblast) transfected with scrambled siRNA control whole cell lysate
Lane 2 : RMS 13 (human rhabdomyosarcoma fibroblast) transfected with siRNA specifically targeting Gli1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 118 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsThis data was developed using ab134906, the same antibody clone in a different buffer formulation.
Diluting and blocking buffer and concentration: 5% NFDM/TBST
This blot was developed using a high sensitivity ECL substrate.
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All lanes : Anti-Gli1 antibody [EPR4523] (ab134906) at 1/1000 dilution
Lane 1 : A673 (human muscle Ewing's Sarcoma) whole cell lysate
Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 3 : RD (Human muscle rhabdomyosarcoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 118 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsThis data was developed using ab134906, the same antibody clone in a different buffer formulation.
Diluting and blocking buffer and concentration: 5% NFDM/TBST
This blot was developed using a high sensitivity ECL substrate.
The expression level of Gli1 in HepG2 and RD are consistent with describes in PMID: 19189974 and PMID: 32493277.
Negative control: HepG2 and RD (PMID: 19189974 and PMID: 32493277).
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Anti-Gli1 antibody [EPR4523] (ab134906) at 1/1000 dilution (purified) + RMS13 cell lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 118 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?This data was developed using ab134906, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Gli1 antibody [EPR4523] (ab134906) at 1/1000 dilution (unpurified)
Lane 1 : RMS13 cell lysate
Lane 2 : A673 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution
Predicted band size: 118 kDaThis data was developed using ab134906, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab240088 has been referenced in 1 publication.
- He Y et al. CircZNF609 enhances hepatocellular carcinoma cell proliferation, metastasis, and stemness by activating the Hedgehog pathway through the regulation of miR-15a-5p/15b-5p and GLI2 expressions. Cell Death Dis 11:358 (2020). PubMed: 32398664