• Product name

  • Description

    Rabbit polyclonal to Gli2
  • Host species

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Chinese hamsterDoes not react with: Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse Gli2 aa 950-1050 conjugated to keyhole limpet haemocyanin.
    Database link: Q0VGT2

  • Positive control

    • WB: Mouse E10 embryonic brain, E12 embryonic brain and spinal cord, E14 embryonic brain, E16 embryonic brain, and E18 embryonic brain tissue lysates.



Our Abpromise guarantee covers the use of ab167389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 167 kDa).
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Acts as a transcriptional activator. May play a role during embryogenesis. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. Implicated in the transduction of SHH signal.
    • Involvement in disease

      Defects in GLI2 are the cause of holoprosencephaly type 9 (HPE9) [MIM:610829]; also called pituitary anomalies with holoprosencephaly-like features. The primary features of this disease include defective anterior pituitary formation and pan-hypopituitarism, with or without overt forebrain cleavage abnormalities, and holoprosencephaly-like midfacial hypoplasia. Holoprosencephaly is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability.
    • Sequence similarities

      Belongs to the GLI C2H2-type zinc-finger protein family.
      Contains 5 C2H2-type zinc fingers.
    • Post-translational

      Phosphorylated in vitro by ULK3.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • CJS antibody
      • Gli 2 antibody
      • GLI family zinc finger 2 antibody
      • GLI Kruppel family member GLI2 antibody
      • GLI2 antibody
      • GLI2_HUMAN antibody
      • Glioma associated oncogene family zinc finger antibody
      • HPE9 antibody
      • Oncogene GLI2 antibody
      • PHS2 antibody
      • Tax helper protein 1 antibody
      • Tax helper protein 2 antibody
      • Tax helper protein antibody
      • Tax responsive element 2 holding protein antibody
      • Tax responsive element 25 bp sequence binding protein antibody
      • THP antibody
      • THP1 antibody
      • THP2 antibody
      • Zinc finger protein GLI2 antibody
      see all


    • All lanes : Anti-Gli2 antibody (ab167389) at 1 µg/ml

      Lane 1 : E10 Mouse Embryo Brain Tissue Lysate
      Lane 2 : E12 Mouse Embryo Brain and Spinal Cord Tissue Lysate
      Lane 3 : E14 Mouse Embryo Brain Tissue Lysate
      Lane 4 : E16 Ms Embryo Brain Tissue Lysate
      Lane 5 : E18 Ms Embryo Brain Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 167 kDa
      Observed band size: 150 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 69 kDa (possible non-specific binding)

      Exposure time: 12 minutes

      This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab167389 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.


    This product has been referenced in:

    • Zhang Y  et al. Vasohibin 2 promotes malignant behaviors of pancreatic cancer cells by inducing epithelial-mesenchymal transition via Hedgehog signaling pathway. Cancer Med 7:5567-5576 (2018). Read more (PubMed: 30318866) »
    • Ye L  et al. NUMB maintains bone mass by promoting degradation of PTEN and GLI1 via ubiquitination in osteoblasts. Bone Res 6:32 (2018). Read more (PubMed: 30455992) »
    See all 6 Publications for this product

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