Overview

  • Product name

  • Description

    Goat polyclonal to Gli2
  • Host species

    Goat
  • Specificity

    This antibody is expected to recognise all four reported isoforms (NP_084655.1, NP_084656.1, NP_084657.1 and NP_005261.1).
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human Gli2 aa 416-429 (internal sequence).
    Sequence:

    DLKEDLDRDDCKQE


    Database link: P10070

  • Positive control

    • IHC: Human lymph node tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab223651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Acts as a transcriptional activator. May play a role during embryogenesis. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. Implicated in the transduction of SHH signal.
  • Involvement in disease

    Defects in GLI2 are the cause of holoprosencephaly type 9 (HPE9) [MIM:610829]; also called pituitary anomalies with holoprosencephaly-like features. The primary features of this disease include defective anterior pituitary formation and pan-hypopituitarism, with or without overt forebrain cleavage abnormalities, and holoprosencephaly-like midfacial hypoplasia. Holoprosencephaly is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability.
  • Sequence similarities

    Belongs to the GLI C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • Post-translational
    modifications

    Phosphorylated in vitro by ULK3.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CJS antibody
    • Gli 2 antibody
    • GLI family zinc finger 2 antibody
    • GLI Kruppel family member GLI2 antibody
    • GLI2 antibody
    • GLI2_HUMAN antibody
    • Glioma associated oncogene family zinc finger antibody
    • HPE9 antibody
    • Oncogene GLI2 antibody
    • PHS2 antibody
    • Tax helper protein 1 antibody
    • Tax helper protein 2 antibody
    • Tax helper protein antibody
    • Tax responsive element 2 holding protein antibody
    • Tax responsive element 25 bp sequence binding protein antibody
    • THP antibody
    • THP1 antibody
    • THP2 antibody
    • Zinc finger protein GLI2 antibody
    see all

Images

  • Paraffin-embedded human lymph node tissue stained for Gli2 using ab223651 at 4 μg/ml in immunohistochemical analysis.

References

This product has been referenced in:

  • Wang N  et al. miR-141-3p suppresses proliferation and promotes apoptosis by targeting GLI2 in osteosarcoma cells. Oncol Rep 39:747-754 (2018). Read more (PubMed: 29251328) »
See 1 Publication for this product

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