Anti-Gli2 antibody (ab226390)

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Key features and details

  • Rabbit polyclonal to Gli2
  • Suitable for: IP
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Gli2 antibody
    See all Gli2 primary antibodies

  • Description

    Rabbit polyclonal to Gli2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IPmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orange

  • Immunogen

    Synthetic peptide within Human Gli2 aa 950-1000. The exact sequence is proprietary.
    Database link: P10070

  • Positive control

    • IP: HeLa cell lysate.

  • General notes

    ab226390 has not performed satisfactorily when used for WB of Gli2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Gli2.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab226390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Acts as a transcriptional activator. May play a role during embryogenesis. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. Implicated in the transduction of SHH signal.

  • Involvement in disease

    Defects in GLI2 are the cause of holoprosencephaly type 9 (HPE9) [MIM:610829]; also called pituitary anomalies with holoprosencephaly-like features. The primary features of this disease include defective anterior pituitary formation and pan-hypopituitarism, with or without overt forebrain cleavage abnormalities, and holoprosencephaly-like midfacial hypoplasia. Holoprosencephaly is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability.

  • Sequence similarities

    Belongs to the GLI C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.

  • Post-translational
    modifications

    Phosphorylated in vitro by ULK3.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • CJS antibody
      • Gli 2 antibody
      • GLI family zinc finger 2 antibody
      • GLI Kruppel family member GLI2 antibody
      • GLI2 antibody
      • GLI2_HUMAN antibody
      • Glioma associated oncogene family zinc finger antibody
      • HPE9 antibody
      • Oncogene GLI2 antibody
      • PHS2 antibody
      • Tax helper protein 1 antibody
      • Tax helper protein 2 antibody
      • Tax helper protein antibody
      • Tax responsive element 2 holding protein antibody
      • Tax responsive element 25 bp sequence binding protein antibody
      • THP antibody
      • THP1 antibody
      • THP2 antibody
      • Zinc finger protein GLI2 antibody
      see all

    Images

    • Immunoprecipitation - Anti-Gli2 antibody (ab226390)
      Immunoprecipitation - Anti-Gli2 antibody (ab226390)

      ARF6 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN buffer; 0.5 or 1.0 mg for IP, 20% of IP loaded) with ab226390 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226390 at 1 µg/ml.

      Lane 1: ab226390 IP in HeLa whole cell lysate.

      Lane 2: Control IgG IP in HeLa whole cell lysate.

      Detection:  Chemiluminescence with exposure time of 30 seconds.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab226390? Please let us know so that we can cite the reference in this datasheet.

    ab226390 has not yet been referenced specifically in any publications.

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