Overview

  • Product name

  • Description

    Rabbit polyclonal to Gli2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orange
  • Immunogen

    Synthetic peptide within Human Gli2 aa 950-1000. The exact sequence is proprietary.
    Database link: P10070

  • Positive control

    • IP: HeLa cell lysate.
  • General notes

    ab226390 has not performed satisfactorily when used for WB of Gli2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Gli2.

Properties

Applications

Our Abpromise guarantee covers the use of ab226390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Acts as a transcriptional activator. May play a role during embryogenesis. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. Implicated in the transduction of SHH signal.
  • Involvement in disease

    Defects in GLI2 are the cause of holoprosencephaly type 9 (HPE9) [MIM:610829]; also called pituitary anomalies with holoprosencephaly-like features. The primary features of this disease include defective anterior pituitary formation and pan-hypopituitarism, with or without overt forebrain cleavage abnormalities, and holoprosencephaly-like midfacial hypoplasia. Holoprosencephaly is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability.
  • Sequence similarities

    Belongs to the GLI C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • Post-translational
    modifications

    Phosphorylated in vitro by ULK3.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CJS antibody
    • Gli 2 antibody
    • GLI family zinc finger 2 antibody
    • GLI Kruppel family member GLI2 antibody
    • GLI2 antibody
    • GLI2_HUMAN antibody
    • Glioma associated oncogene family zinc finger antibody
    • HPE9 antibody
    • Oncogene GLI2 antibody
    • PHS2 antibody
    • Tax helper protein 1 antibody
    • Tax helper protein 2 antibody
    • Tax helper protein antibody
    • Tax responsive element 2 holding protein antibody
    • Tax responsive element 25 bp sequence binding protein antibody
    • THP antibody
    • THP1 antibody
    • THP2 antibody
    • Zinc finger protein GLI2 antibody
    see all

Images

  • ARF6 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN buffer; 0.5 or 1.0 mg for IP, 20% of IP loaded) with ab226390 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226390 at 1 µg/ml.

    Lane 1: ab226390 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 30 seconds.

References

ab226390 has not yet been referenced specifically in any publications.

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