Anti-Gli2 antibody (ab226390)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Gli2 antibody
See all Gli2 primary antibodies -
Description
Rabbit polyclonal to Gli2 -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orange
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Immunogen
Synthetic peptide within Human Gli2 aa 950-1000. The exact sequence is proprietary.
Database link: P10070 -
Positive control
- IP: HeLa cell lysate.
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General notes
ab226390 has not performed satisfactorily when used for WB of Gli2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Gli2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab226390 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Acts as a transcriptional activator. May play a role during embryogenesis. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. Implicated in the transduction of SHH signal. -
Involvement in disease
Defects in GLI2 are the cause of holoprosencephaly type 9 (HPE9) [MIM:610829]; also called pituitary anomalies with holoprosencephaly-like features. The primary features of this disease include defective anterior pituitary formation and pan-hypopituitarism, with or without overt forebrain cleavage abnormalities, and holoprosencephaly-like midfacial hypoplasia. Holoprosencephaly is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. -
Sequence similarities
Belongs to the GLI C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. -
Post-translational
modificationsPhosphorylated in vitro by ULK3. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2736 Human
- Omim: 165230 Human
- SwissProt: P10070 Human
- Unigene: 111867 Human
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Alternative names
- CJS antibody
- Gli 2 antibody
- GLI family zinc finger 2 antibody
see all
Images
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Immunoprecipitation - Anti-Gli2 antibody (ab226390)ARF6 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN buffer; 0.5 or 1.0 mg for IP, 20% of IP loaded) with ab226390 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226390 at 1 µg/ml.
Lane 1: ab226390 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Datasheets and documents
References
ab226390 has not yet been referenced specifically in any publications.
