Anti-Gli2 antibody [OTI1F9] (ab187386)
Key features and details
- Mouse monoclonal [OTI1F9] to Gli2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Gli2 antibody [OTI1F9]
See all Gli2 primary antibodies -
Description
Mouse monoclonal [OTI1F9] to Gli2 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Gli2 aa 700-1150. Produced in E.coli. (NP_005261).
Database link: P10070-5 -
Positive control
- WB: HEK293T cell lysate transfected with pCMV6-ENTRY Gli2 cDNA. IHC-P: Human skin tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography -
Clonality
Monoclonal -
Clone number
OTI1F9 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500. Predicted molecular weight: 167 kDa.
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IHC-P |
1/150.
Perform heat mediated antigen retrieval with Tris-EDTA, pH8.0 before commencing with IHC staining protocol. |
Notes |
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WB
1/500. Predicted molecular weight: 167 kDa. |
IHC-P
1/150. Perform heat mediated antigen retrieval with Tris-EDTA, pH8.0 before commencing with IHC staining protocol. |
Target
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Function
Acts as a transcriptional activator. May play a role during embryogenesis. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. Implicated in the transduction of SHH signal. -
Involvement in disease
Defects in GLI2 are the cause of holoprosencephaly type 9 (HPE9) [MIM:610829]; also called pituitary anomalies with holoprosencephaly-like features. The primary features of this disease include defective anterior pituitary formation and pan-hypopituitarism, with or without overt forebrain cleavage abnormalities, and holoprosencephaly-like midfacial hypoplasia. Holoprosencephaly is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. -
Sequence similarities
Belongs to the GLI C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. -
Post-translational
modificationsPhosphorylated in vitro by ULK3. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2736 Human
- Omim: 165230 Human
- SwissProt: P10070 Human
- Unigene: 111867 Human
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Alternative names
- CJS antibody
- Gli 2 antibody
- GLI family zinc finger 2 antibody
see all
Images
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All lanes : Anti-Gli2 antibody [OTI1F9] (ab187386) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY Gli2 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 167 kDa -
Paraffin-embedded Human skin tissue labeling Gli2 with ab187386 at 1/150 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab187386 has been referenced in 6 publications.
- Liu X et al. MUC21 controls melanoma progression via regulating SLITRK5 and hedgehog signaling pathway. Cell Biol Int 46:1458-1467 (2022). PubMed: 35579188
- Ichimiya S et al. GLI2 but not GLI1/GLI3 plays a central role in the induction of malignant phenotype of gallbladder cancer. Oncol Rep 45:997-1010 (2021). PubMed: 33650666
- Peng Y et al. Dual activation of Hedgehog and Wnt/ß-catenin signaling pathway caused by downregulation of SUFU targeted by miRNA-150 in human gastric cancer. Aging (Albany NY) 13:10749-10769 (2021). PubMed: 33848981
- Ye G et al. Circ_DCAF6 potentiates cell stemness and growth in breast cancer through GLI1-Hedgehog pathway. Exp Mol Pathol 116:104492 (2020). PubMed: 32668286
- Hua J et al. Small Molecule-Based Strategy Promotes Nucleus Pulposus Specific Differentiation of Adipose-Derived Mesenchymal Stem Cells. Mol Cells 42:661-671 (2019). PubMed: 31564076
- Zhang CY et al. Saikosaponin-d Inhibits the Hepatoma Cells and Enhances Chemosensitivity Through SENP5-Dependent Inhibition of Gli1 SUMOylation Under Hypoxia. Front Pharmacol 10:1039 (2019). PubMed: 31616295