Key features and details
- Mouse monoclonal [OTI1F9] to Gli2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Gli2 antibody [OTI1F9]
See all Gli2 primary antibodies
DescriptionMouse monoclonal [OTI1F9] to Gli2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Gli2 aa 729-1123. Produced in E.coli. (NP_005261).
FEQLKKEKLKSLKDSCSWAGPTPHTRNTKLPPLPGSGSILENFSGSGGGG PAGLLPNPRLSELSASEVTMLSQLQERRDSSTSTVSSAYTVSRRSSGISP YFSSRRSSEASPLGAGRPHNASSADSYDPISTDASRRSSEASQCSGGSGL LNLTPAQQYSLRAKYAAATGGPPPTPLPGLERMSLRTRLALLDAPERTLP AGCPRPLGPRRGSDGPTYGHGHAGAAPAFPHEAPGGGARRASDPVRRPDA LSLPRVQRFHSTHNVNPGPLPPCADRRGLRLQSHPSTDGGLARGAYSPRP PSISENVAMEAVAAGVDGAGPEADLGLPEDDLVLPDDVVQYIKAHASGAL DEGTGQVYPTESTGFSDNPRLPSPGLHGQRRMVAADSNVGPSAPM
Database link: P10070
- WB: HEK293T cell lysate transfected with pCMV6-ENTRY Gli2 cDNA. IHC-P: Human skin tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 1% BSA
Concentration information loading...
Purification notesPurified from cell culture supernatant by affinity chromatography
Our Abpromise guarantee covers the use of ab187386 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Predicted molecular weight: 167 kDa.|
Perform heat mediated antigen retrieval with Tris-EDTA, pH8.0 before commencing with IHC staining protocol.
FunctionActs as a transcriptional activator. May play a role during embryogenesis. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. Implicated in the transduction of SHH signal.
Involvement in diseaseDefects in GLI2 are the cause of holoprosencephaly type 9 (HPE9) [MIM:610829]; also called pituitary anomalies with holoprosencephaly-like features. The primary features of this disease include defective anterior pituitary formation and pan-hypopituitarism, with or without overt forebrain cleavage abnormalities, and holoprosencephaly-like midfacial hypoplasia. Holoprosencephaly is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability.
Sequence similaritiesBelongs to the GLI C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers.
modificationsPhosphorylated in vitro by ULK3.
- Information by UniProt
- CJS antibody
- Gli 2 antibody
- GLI family zinc finger 2 antibody
All lanes : Anti-Gli2 antibody [OTI1F9] (ab187386) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY Gli2 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 167 kDa
Paraffin-embedded Human skin tissue labeling Gli2 with ab187386 at 1/150 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab187386 has been referenced in 2 publications.
- Hua J et al. Small Molecule-Based Strategy Promotes Nucleus Pulposus Specific Differentiation of Adipose-Derived Mesenchymal Stem Cells. Mol Cells 42:661-671 (2019). PubMed: 31564076
- Zhang CY et al. Saikosaponin-d Inhibits the Hepatoma Cells and Enhances Chemosensitivity Through SENP5-Dependent Inhibition of Gli1 SUMOylation Under Hypoxia. Front Pharmacol 10:1039 (2019). PubMed: 31616295