Recombinant Anti-Gli3 antibody [EPR4594] (ab181130)
![Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (ab181130)](https://www.abcam.com/ps/products/181/ab181130/Images/ab181130-449331-AntiGli3-antibody-ICC.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (ab181130)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4594] to Gli3
- Suitable for: ICC/IF, WB
- Reacts with: Human
Overview
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Product name
Anti-Gli3 antibody [EPR4594]
See all Gli3 primary antibodies -
Description
Rabbit monoclonal [EPR4594] to Gli3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee
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Immunogen
Recombinant fragment within Human Gli3 aa 1300-1500. The exact sequence is proprietary.
Database link: P10071 -
Positive control
- WB: 293 lysate ICC/IF: 293 cells and human kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4594 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab181130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100. | |
| WB | 1/1000 - 1/2000. Predicted molecular weight: 190 kDa. |
Target
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Function
Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'. -
Tissue specificity
Is expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. -
Involvement in disease
Defects in GLI3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS) [MIM:175700]. GCPS is an autosomal dominant disorder affecting limb and craniofacial development. It is characterized by pre- and postaxial polydactyly, syndactyly of fingers and toes, macrocephaly and hypertelorism.
Defects in GLI3 are a cause of Pallister-Hall syndrome (PHS) [MIM:146510]. PHS is characterized by a wide range of clinical manifestations. It mainly associates central or postaxial polydactyly, syndactyly, and hypothalamic hamartoma. Malformations are frequent in the viscera, e.g. anal atresia, bifid uvula, congenital heart malformations, pulmonary or renal dysplasia. It is an autosomal dominant disorder.
Defects in GLI3 are a cause of type A1/B postaxial polydactyly (PAPA1/PAPB) [MIM:174200, 603596]. PAPA in humans is an autosomal dominant trait characterized by an extra digit in the ulnar and/or fibular side of the upper and/or lower extremities. The extra digit is well formed and articulates with the fifth, or extra, metacarpal/metatarsal, and thus it is usually functional.
Defects in GLI3 are a cause of polydactyly preaxial type 4 (POP4) [MIM:174700]. Polydactyly preaxial type 4 (i.e., polydactyly on the radial/tibial side of the hand/foot) covers a heterogeneous group of entities. In preaxial polydactyly type IV, the thumb shows only the mildest degree of duplication, and syndactyly of various degrees affects fingers 3 and 4.
Defects in GLI3 are the cause of acrocallosal syndrome (ACS) [MIM:200990]; also abbreviated ACLS. ACS is characterized by postaxial polydactyly, hallux duplication, macrocephaly, and absence of the corpus callosum, usually with severe developmental delay. -
Sequence similarities
Belongs to the GLI C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. -
Post-translational
modificationsPhosphorylated on multiple sites by protein kinase A (PKA) and phosphorylation by PKA primes further phosphorylation by CK1 and GSK3. Phosphorylation is essential for its proteolytic processing.
Transcriptional repressor GLI3R, a C-terminally truncated form, is generated from the full-length GLI3 protein (GLI3FL/GLI3-190) through proteolytic processing. This process requires PKA-primed phosphorylation of GLI3, ubiquitination of GLI3 and the presence of BTRC. GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state. Without the Hh signal, the SUFU-GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R. GLI3R formation leads to its dissociation from SUFU, allowing it to translocate into the nucleus, and repress Hh target genes. When Hh signaling is initiated, SUFU dissociates from GLI3FL and this has two consequences. First, GLI3R production is halted. Second, free GLI3FL translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A). Phosphorylated in vitro by ULK3. -
Cellular localization
Nucleus. Cytoplasm. Cell projection > cilium. GLI3FL is localized predominantly in the cytoplasm while GLI3R resides mainly in the nucleus. Ciliary accumulation requires the presence of KIF7 and SMO. Translocation to the nucleus is promoted by interaction with ZIC1. - Information by UniProt
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Database links
- Entrez Gene: 463369 Chimpanzee
- Entrez Gene: 2737 Human
- Omim: 165240 Human
- SwissProt: Q5IS56 Chimpanzee
- SwissProt: P10071 Human
- Unigene: 21509 Human
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Alternative names
- ACLS antibody
- DNA binding protein antibody
- GCPS antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (ab181130)This image is courtesy of an abreview by Lindsey Fitzsimons
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue labelling Gli3 with ab181130 at 1/100 dilution. Antigen retrieval was performed using Tris/EDTA buffer pH 9 for 30 minutes, a HRP-conjugated goat anti-rabbit IgG (H+L) (Alexa Fluor® 488) ab150078 was used as the secondary antibody (1/1000). Sections were co-stained with Monoclonal Anti-tubulin. Magnification at x100
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![Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (ab181130)](https://www.abcam.com/ps/products/181/ab181130/Images/ab181130-449330-AntiGli3-antibody-ICC.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (ab181130)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue labelling Gli3 with ab181130 at 1/100 dilution. Antigen retrieval was performed using Tris/EDTA buffer pH 9 for 30 minutes, a HRP-conjugated goat anti-rabbit IgG (H+L) (Alexa Fluor® 488) ab150078 was used as the secondary antibody (1/1000). Sections were co-stained with Monoclonal Anti-tubulin. Magnification at x40.
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![Western blot - Anti-Gli3 antibody [EPR4594] (ab181130)](https://www.abcam.com/ps/products/181/ab181130/Images/ab181130-213441-anti-gli3-antibody-epr4594-western-blot.jpg)
Western blot - Anti-Gli3 antibody [EPR4594] (ab181130)Anti-Gli3 antibody [EPR4594] (ab181130) at 1/1000 dilution + 293 lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 190 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (ab181130)](https://www.abcam.com/ps/products/181/ab181130/Images/ab181130-213442-anti-gli3-antibody-epr4594-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody [EPR4594] (ab181130)Immunofluorescence analysis of 293 cells (fixative 4% paraformaldehyde) labeling Gli3 with ab181130 at a 1/100 dilution. Goat anti rabbit IgG (Alexa Fluor 488™) secondary used at a 1/200 diution.
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![Anti-Gli3 antibody [EPR4594] (ab181130)](https://www.abcam.com/ps/products/181/ab181130/Images/ab181130-3-benefits-of-recombinant-antibodies.png)
Anti-Gli3 antibody [EPR4594] (ab181130)
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab181130 has not yet been referenced specifically in any publications.
