Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human prostate cancer cell)
Permeabilization
Yes - PBS with 0.025%Triton
Specification
Human prostate cancer cell
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Methanol
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS with 0.025% Triton
Dilution
1/200
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Goat Anti Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Additional data
Additional Notes
Human prostate cancer cells: LNCaP cell were used to test the Ab.
Abcam user community
Verified customer
Submitted Jul 27 2015