Product nameAnti-Glicentin antibody
See all Glicentin primary antibodies
DescriptionRabbit polyclonal to Glicentin
SpecificityThis glicentin antibody is reactive with glucagon as well as glicentin. Glicentin contains the glucagon sequence and is produced in endocrine cells of the distal intestine, in pancreatic glucagon cells and in nerves in the brain.
Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, Dot blotmore details
Species reactivityReacts with: Rat, Human
Porcine pancreatic Glucagon/BSA
- Pancreas and small intestine
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferLiquid antiserum
Light chain typeunknown
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab8493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This antibody is suitable for paraffin embedded tissues without enzymatic antigen retrieval.
Advised working dilution is 1:50.
Dilution buffer is 0.15 M PBS with 1.0% Ovalbumin and 0.1% sodium azide.
Optimal dilution should be tested by serial dilution.
FunctionGlucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.
GLP-1 is a potent stimulator of glucose-dependent insulin release. Play important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Have growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferaton. Inhibits beta cell apoptosis.
GLP-2 stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability.
Oxyntomodulin significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness.
Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
Tissue specificityGlucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain.
Sequence similaritiesBelongs to the glucagon family.
modificationsProglucagon is post-translationally processed in a tissue-specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.
- Information by UniProt
FormCleaved into the following 8 chains: 1.Glicentin; 2.Glicentin-related polypeptide = GRPP; 3.Oxyntomodulin = OXM = OXY; 4.Glucagon; 5.Glucagon-like peptide 1 = GLP-1; 6.Glucagon-like peptide 1(7-37) = GLP-1(7-37); 7.Glucagon-like peptide 1(7-36) = GLP-1(7-36); 8.Glucagon-like peptide 2 = GLP-2.
- GCG antibody
- glicentin-related polypeptide antibody
- GLP-1 antibody
ab8493 has not yet been referenced specifically in any publications.