Product nameAnti-GLO1 antibody
See all GLO1 primary antibodies
DescriptionRabbit polyclonal to GLO1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Cow, Chimpanzee, Macaque monkey, Orangutan
Synthetic peptide corresponding to Human GLO1 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Human Heart and Brain tissue lysates as well as the following whole cell lysates: HepG2; HeLa; HEK293; A431. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab129124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 21 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionCatalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.
PathwaySecondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.
Sequence similaritiesBelongs to the glyoxalase I family.
- Information by UniProt
- Aldoketomutase antibody
- glo1 antibody
- GLOD1 antibody
ICC/IF image of ab129124 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab129124 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-GLO1 antibody (ab129124) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Human brain tissue lysate - total protein (ab29466)
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 6 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa, 64 kDa, 98 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
The predicted molecular weight of GLO1 is 21 kDa (SwissProt), however we expect to observe a banding pattern around 25 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129124 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab129124 has not yet been referenced specifically in any publications.