Product nameAnti-GLO1 antibody
See all GLO1 primary antibodies
DescriptionRabbit polyclonal to GLO1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Cow
Recombinant protein fragment corresponding to a region within amino acids 1 and 171 of GLO1 (NP_006699).
- WB: 293T, A431, H1299, HeLaS3, HepG2, MOLT and Raji cell lysates IHC-P: ovarian carcinoma tissue ICC/IF: HeLa cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 21 kDa.|
|IHC-P||1/100 - 1/250.|
|ICC/IF||1/100 - 1/200.|
FunctionCatalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.
PathwaySecondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.
Sequence similaritiesBelongs to the glyoxalase I family.
- Information by UniProt
- Aldoketomutase antibody
- glo1 antibody
- GLOD1 antibody
All lanes : Anti-GLO1 antibody (ab96032) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lane 5 : HepG2 whole cell lysate
Lane 6 : MOLT4 whole cell lysate
Lane 7 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 21 kDa
12% SDS PAGE
Immunohistochemical analysis of GLO1 in paraffin embedded human ovarian carcinoma tissue, using ab96032 at a 1/100 dilution.
Immunofluorescence analysis of GLO1 in paraformaldehyde fixed HeLa, using ab96032 at a 1/200 dilution. Lower image: merged with DNA probe.
This product has been referenced in:
- Spanos C et al. Proteomic identification and characterization of hepatic glyoxalase 1 dysregulation in non-alcoholic fatty liver disease. Proteome Sci 16:4 (2018). Read more (PubMed: 29456458) »
- Rodrigues T et al. Methylglyoxal-induced glycation changes adipose tissue vascular architecture, flow and expansion, leading to insulin resistance. Sci Rep 7:1698 (2017). WB ; Rat . Read more (PubMed: 28490763) »