Overview

  • Product name

  • Description

    Rabbit polyclonal to GLO1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 and 171 of GLO1 (NP_006699).

  • Positive control

    • WB: 293T, A431, H1299, HeLaS3, HepG2, MOLT and Raji cell lysates IHC-P: ovarian carcinoma tissue ICC/IF: HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab96032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 21 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.
  • Pathway

    Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.
  • Sequence similarities

    Belongs to the glyoxalase I family.
  • Information by UniProt
  • Database links

  • Alternative names

    • Aldoketomutase antibody
    • glo1 antibody
    • GLOD1 antibody
    • Glx I antibody
    • GLYI antibody
    • glyoxalase domain containing 1 antibody
    • Glyoxalase I antibody
    • Ketone aldehyde mutase antibody
    • Ketone-aldehyde mutase antibody
    • Lactoyl glutathione lyase antibody
    • Lactoylglutathione lyase antibody
    • LGUL_HUMAN antibody
    • Methylglyoxalase antibody
    • S D lactoylglutathione methylglyoxal lyase antibody
    • S-D-lactoylglutathione methylglyoxal lyase antibody
    see all

Images

  • All lanes : Anti-GLO1 antibody (ab96032) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate
    Lane 4 : HeLa whole cell lysate
    Lane 5 : HepG2 whole cell lysate
    Lane 6 : MOLT4 whole cell lysate
    Lane 7 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 21 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of GLO1 in paraffin embedded human ovarian carcinoma tissue, using ab96032 at a 1/100 dilution.

  • Immunofluorescence analysis of GLO1 in paraformaldehyde fixed HeLa, using ab96032 at a 1/200 dilution. Lower image: merged with DNA probe.

References

This product has been referenced in:

  • Spanos C  et al. Proteomic identification and characterization of hepatic glyoxalase 1 dysregulation in non-alcoholic fatty liver disease. Proteome Sci 16:4 (2018). Read more (PubMed: 29456458) »
  • Rodrigues T  et al. Methylglyoxal-induced glycation changes adipose tissue vascular architecture, flow and expansion, leading to insulin resistance. Sci Rep 7:1698 (2017). WB ; Rat . Read more (PubMed: 28490763) »
See all 7 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Sample
Rat Tissue sections (DRG)
Specification
DRG
Permeabilization
No
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Aug 22 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Rat Tissue sections (DRG)
Specification
DRG
Permeabilization
No
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Aug 20 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Ovarian carcinoma)
Specification
Ovarian carcinoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric Acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Mr. Carl Hobbs

Verified customer

Submitted Oct 19 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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