Recombinant
RabMAb

Recombinant Anti-GLO1 antibody [EPR8878] (ab137098)

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Overview

  • Product name

    Anti-GLO1 antibody [EPR8878]
    See all GLO1 primary antibodies

  • Description

    Rabbit monoclonal [EPR8878] to GLO1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human GLO1 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Molt-4, Raji, TF-1, HeLa, and red blood cell lysates; Molt-4 cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.

    • Target

    • Function

      Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.

    • Pathway

      Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.

    • Sequence similarities

      Belongs to the glyoxalase I family.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Aldoketomutase antibody
      • glo1 antibody
      • GLOD1 antibody
      • Glx I antibody
      • GLYI antibody
      • glyoxalase domain containing 1 antibody
      • Glyoxalase I antibody
      • Ketone aldehyde mutase antibody
      • Ketone-aldehyde mutase antibody
      • Lactoyl glutathione lyase antibody
      • Lactoylglutathione lyase antibody
      • LGUL_HUMAN antibody
      • Methylglyoxalase antibody
      • S D lactoylglutathione methylglyoxal lyase antibody
      • S-D-lactoylglutathione methylglyoxal lyase antibody
      see all

    Images

    • Western blot - Anti-GLO1 antibody [EPR8878] (ab137098)
      Western blot - Anti-GLO1 antibody [EPR8878] (ab137098)
      All lanes : Anti-GLO1 antibody [EPR8878] (ab137098) at 1/1000 dilution

      Lane 1 : Molt-4 cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : TF-1 cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : Red blood cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 21 kDa

    • Flow Cytometry - Anti-GLO1 antibody [EPR8878] (ab137098)
      Flow Cytometry - Anti-GLO1 antibody [EPR8878] (ab137098)
      Flow cytometric analysis of permeabilized Molt-4 cells labelling GLO1 with ab137098 at 1/10 dilution (red). Negative Rabbit IgG shown in green.

    References

    ab137098 has not yet been referenced specifically in any publications.

    Publishing research using ab137098? Please let us know so that we can cite the reference in this datasheet.

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