Recombinant Anti-GLO1 antibody [EPR8878] (ab137098)
![Western blot - Anti-GLO1 antibody [EPR8878] (ab137098)](https://www.abcam.com/ps/products/137/ab137098/Images/ab137098-356638-AntiGLO1antibodyEPR8878westernblotknockoutHAP1GLO1HeLaHepG2humanlysates.jpg "Western blot - Anti-GLO1 antibody [EPR8878] (ab137098)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8878] to GLO1
- Suitable for: WB, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-GLO1 antibody [EPR8878]
See all GLO1 primary antibodies -
Description
Rabbit monoclonal [EPR8878] to GLO1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide within Human GLO1 aa 1-100. The exact sequence is proprietary.
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Positive control
- Molt-4, Raji, TF-1, HeLa, and red blood cell lysates; Molt-4 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8878 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137098 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
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| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa. |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. -
Pathway
Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2. -
Sequence similarities
Belongs to the glyoxalase I family. - Information by UniProt
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Database links
- Entrez Gene: 2739 Human
- Entrez Gene: 109801 Mouse
- Entrez Gene: 294320 Rat
- Omim: 138750 Human
- SwissProt: Q04760 Human
- SwissProt: Q9CPU0 Mouse
- SwissProt: Q6P7Q4 Rat
- Unigene: 268849 Human
see all -
Alternative names
- Aldoketomutase antibody
- glo1 antibody
- GLOD1 antibody
see all
Images
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Western blot - Anti-GLO1 antibody [EPR8878] (ab137098)All lanes : Anti-GLO1 antibody [EPR8878] (ab137098) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : GLO1 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 21 kDaLanes 1 - 4: Merged signal (red and green). Green - ab137098 observed at 21 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab137098 was shown to specifically react with in wild-type HAP1 cells as signal was lost in GLO1 knockout cells. Wild-type and GLO1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab137098 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-GLO1 antibody [EPR8878] (ab137098)](https://www.abcam.com/ps/products/137/ab137098/Images/ab137098-151694-anti-glo1-antibody-epr8878-western-blot.jpg)
Western blot - Anti-GLO1 antibody [EPR8878] (ab137098)All lanes : Anti-GLO1 antibody [EPR8878] (ab137098) at 1/1000 dilution
Lane 1 : Molt-4 cell lysate
Lane 2 : Raji cell lysate
Lane 3 : TF-1 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : Red blood cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 21 kDa -
![Flow Cytometry (Intracellular) - Anti-GLO1 antibody [EPR8878] (ab137098)](https://www.abcam.com/ps/products/137/ab137098/Images/ab137098-151693-anti-glo1-antibody-epr8878-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-GLO1 antibody [EPR8878] (ab137098)Intracellular flow cytometric analysis of permeabilized Molt-4 cells labelling GLO1 with ab137098 at 1/10 dilution (red). Negative Rabbit IgG shown in green.
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![Anti-GLO1 antibody [EPR8878] (ab137098)](https://www.abcam.com/ps/products/137/ab137098/Images/ab137098-4-benefits-of-recombinant-antibodies.png)
Anti-GLO1 antibody [EPR8878] (ab137098)
Datasheets and documents
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Datasheet download
References (5)
ab137098 has been referenced in 5 publications.
- Prantner D et al. Classically activated mouse macrophages produce methylglyoxal that induces a TLR4- and RAGE-independent proinflammatory response. J Leukoc Biol 109:605-619 (2021). PubMed: 32678947
- Shinozuka K et al. Empathy in stroke rats is modulated by social settings. J Cereb Blood Flow Metab 40:1182-1192 (2020). PubMed: 31366299
- Kulkarni CA et al. ALKBH7 mediates necrosis via rewiring of glyoxal metabolism. Elife 9:N/A (2020). PubMed: 32795389
- Morgenstern J et al. Phosphorylation of T107 by CamKIId Regulates the Detoxification Efficiency and Proteomic Integrity of Glyoxalase 1. Cell Rep 32:108160 (2020). PubMed: 32966793
- Tang M et al. Hippocampal proteomic changes of susceptibility and resilience to depression or anxiety in a rat model of chronic mild stress. Transl Psychiatry 9:260 (2019). PubMed: 31624233
