For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    glo1-antibody-epr8878-ab137098.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Metabolism Energy Metabolism
Share by email
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-GLO1 antibody [EPR8878] (ab137098)

  • Datasheet
Submit a review Submit a question References (5)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-GLO1 antibody [EPR8878] (ab137098)
  • Western blot - Anti-GLO1 antibody [EPR8878] (ab137098)
  • Flow Cytometry (Intracellular) - Anti-GLO1 antibody [EPR8878] (ab137098)
  • Anti-GLO1 antibody [EPR8878] (ab137098)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8878] to GLO1
  • Suitable for: WB, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Primary
Product image
Anti-TLR4 antibody (ab13556)
Assay
Product image
Glyoxalase I Assay Kit (Colorimetric) (ab241019)
Protein
Product image
Recombinant human GLO1 protein (ab87413)

View more associated products

Overview

  • Product name

    Anti-GLO1 antibody [EPR8878]
    See all GLO1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8878] to GLO1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt (Intra)more details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human GLO1 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Molt-4, Raji, TF-1, HeLa, and red blood cell lysates; Molt-4 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8878
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • Other
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Alternative Versions

    • Anti-GLO1 antibody [EPR8878] - BSA and Azide free (ab248817)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
    • MOLT-4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant human GLO1 protein (ab87413)
  • Related Products

    • Recombinant human GLO1 protein (ab87413)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes
Is unsuitable for ICC/IF,IHC-P or IP.

Target

  • Function

    Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.
  • Pathway

    Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.
  • Sequence similarities

    Belongs to the glyoxalase I family.
  • Target information above from: UniProt accession Q04760 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2739 Human
    • Entrez Gene: 109801 Mouse
    • Entrez Gene: 294320 Rat
    • Omim: 138750 Human
    • SwissProt: Q04760 Human
    • SwissProt: Q9CPU0 Mouse
    • SwissProt: Q6P7Q4 Rat
    • Unigene: 268849 Human
    • Unigene: 261984 Mouse
    • Unigene: 108014 Rat
    see all
  • Alternative names

    • Aldoketomutase antibody
    • glo1 antibody
    • GLOD1 antibody
    • Glx I antibody
    • GLYI antibody
    • glyoxalase domain containing 1 antibody
    • Glyoxalase I antibody
    • Ketone aldehyde mutase antibody
    • Ketone-aldehyde mutase antibody
    • Lactoyl glutathione lyase antibody
    • Lactoylglutathione lyase antibody
    • LGUL_HUMAN antibody
    • Methylglyoxalase antibody
    • S D lactoylglutathione methylglyoxal lyase antibody
    • S-D-lactoylglutathione methylglyoxal lyase antibody
    see all

Images

  • Western blot - Anti-GLO1 antibody [EPR8878] (ab137098)
    Western blot - Anti-GLO1 antibody [EPR8878] (ab137098)
    All lanes : Anti-GLO1 antibody [EPR8878] (ab137098) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : GLO1 knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 21 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab137098 observed at 21 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab137098 was shown to specifically react with in wild-type HAP1 cells as signal was lost in GLO1 knockout cells. Wild-type and GLO1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab137098 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-GLO1 antibody [EPR8878] (ab137098)
    Western blot - Anti-GLO1 antibody [EPR8878] (ab137098)
    All lanes : Anti-GLO1 antibody [EPR8878] (ab137098) at 1/1000 dilution

    Lane 1 : Molt-4 cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : TF-1 cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : Red blood cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution

    Predicted band size: 21 kDa

  • Flow Cytometry (Intracellular) - Anti-GLO1 antibody [EPR8878] (ab137098)
    Flow Cytometry (Intracellular) - Anti-GLO1 antibody [EPR8878] (ab137098)

    Intracellular flow cytometric analysis of permeabilized Molt-4 cells labelling GLO1 with ab137098 at 1/10 dilution (red). Negative Rabbit IgG shown in green.

     

  • Anti-GLO1 antibody [EPR8878] (ab137098)
    Anti-GLO1 antibody [EPR8878] (ab137098)

Protocols

  • Western blot protocols
  • Flow cytometry protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (5)

Publishing research using ab137098? Please let us know so that we can cite the reference in this datasheet.

ab137098 has been referenced in 5 publications.

  • Prantner D  et al. Classically activated mouse macrophages produce methylglyoxal that induces a TLR4- and RAGE-independent proinflammatory response. J Leukoc Biol 109:605-619 (2021). PubMed: 32678947
  • Shinozuka K  et al. Empathy in stroke rats is modulated by social settings. J Cereb Blood Flow Metab 40:1182-1192 (2020). PubMed: 31366299
  • Kulkarni CA  et al. ALKBH7 mediates necrosis via rewiring of glyoxal metabolism. Elife 9:N/A (2020). PubMed: 32795389
  • Morgenstern J  et al. Phosphorylation of T107 by CamKIId Regulates the Detoxification Efficiency and Proteomic Integrity of Glyoxalase 1. Cell Rep 32:108160 (2020). PubMed: 32966793
  • Tang M  et al. Hippocampal proteomic changes of susceptibility and resilience to depression or anxiety in a rat model of chronic mild stress. Transl Psychiatry 9:260 (2019). PubMed: 31624233

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab137098.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.