Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-GLO1 antibody [EPR8878] (ab137098)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8878] to GLO1
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GLO1 antibody [EPR8878]
    See all GLO1 primary antibodies

  • Description

    Rabbit monoclonal [EPR8878] to GLO1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human GLO1 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Molt-4, Raji, TF-1, HeLa, and red blood cell lysates; Molt-4 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab137098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.

    • Target

    • Function

      Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.

    • Pathway

      Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.

    • Sequence similarities

      Belongs to the glyoxalase I family.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Aldoketomutase antibody
      • glo1 antibody
      • GLOD1 antibody
      • Glx I antibody
      • GLYI antibody
      • glyoxalase domain containing 1 antibody
      • Glyoxalase I antibody
      • Ketone aldehyde mutase antibody
      • Ketone-aldehyde mutase antibody
      • Lactoyl glutathione lyase antibody
      • Lactoylglutathione lyase antibody
      • LGUL_HUMAN antibody
      • Methylglyoxalase antibody
      • S D lactoylglutathione methylglyoxal lyase antibody
      • S-D-lactoylglutathione methylglyoxal lyase antibody
      see all

    Images

    • Western blot - Anti-GLO1 antibody [EPR8878] (ab137098)
      Western blot - Anti-GLO1 antibody [EPR8878] (ab137098)
      All lanes : Anti-GLO1 antibody [EPR8878] (ab137098) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : GLO1 knockout HAP1 whole cell lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : HepG2 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 21 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab137098 observed at 21 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab137098 was shown to specifically react with in wild-type HAP1 cells as signal was lost in GLO1 knockout cells. Wild-type and GLO1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab137098 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-GLO1 antibody [EPR8878] (ab137098)
      Western blot - Anti-GLO1 antibody [EPR8878] (ab137098)
      All lanes : Anti-GLO1 antibody [EPR8878] (ab137098) at 1/1000 dilution

      Lane 1 : Molt-4 cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : TF-1 cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : Red blood cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 21 kDa

    • Flow Cytometry - Anti-GLO1 antibody [EPR8878] (ab137098)
      Flow Cytometry - Anti-GLO1 antibody [EPR8878] (ab137098)
      Flow cytometric analysis of permeabilized Molt-4 cells labelling GLO1 with ab137098 at 1/10 dilution (red). Negative Rabbit IgG shown in green.

    References

    Publishing research using ab137098? Please let us know so that we can cite the reference in this datasheet.

    ab137098 has been referenced in 1 publication.

    • Tang M  et al. Hippocampal proteomic changes of susceptibility and resilience to depression or anxiety in a rat model of chronic mild stress. Transl Psychiatry 9:260 (2019). PubMed: 31624233

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