Product nameAnti-GLO1 antibody [Glo1a]
See all GLO1 primary antibodies
DescriptionMouse monoclonal [Glo1a] to GLO1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human, Monkey
Recombinant full length protein (proprietary-tag) corresponding to Human GLO1 aa 1-184.
MAEPQPPSGGLTDEAALSCCSDADPSTKDFLLQQTMLRVKDPKKSLDFYT RVLGMTLIQKCDFPIMKFSLYFLAYEDKNDIPKEKDEKIAWALSRKATLE LTHNWGTEDDETQSYHNGNSDPRGFGHIGIAVPDVYSACKRFEELGVKFV KKPDDGKMKGLAFIQDPDGYWIEILNPNKMATLM
Database link: Q04760
- HeLa, HepG2, 293T, A431, A549, MCF7, U2OS, K562, monkey COS7, mouse MEF, mouse C2C12 and rat NRK cell lysates; Human prostate and stomach cancer tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
Concentration information loading...
PurityProtein A purified
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- HeLa whole cell lysate (ab29545)
- MCF7 whole cell lysate (ab3871)
- C2C12 whole cell lysate (ab7182)
- HepG2 whole cell lysate (ab7900)
- A431 whole cell lysate (ab7909)
- A549 whole cell lysate (ab7910)
- K562 whole cell lysate (ab7911)
- 293T whole cell lysate (ab95494)
Our Abpromise guarantee covers the use of ab171121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 21 kDa.|
|IHC-P||1/800. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionCatalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.
PathwaySecondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.
Sequence similaritiesBelongs to the glyoxalase I family.
- Information by UniProt
- Aldoketomutase antibody
- glo1 antibody
- GLOD1 antibody
All lanes : Anti-GLO1 antibody [Glo1a] (ab171121) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : A431 cell lysate
Lane 5 : A549 cell lysate
Lane 6 : MCF7 cell lysate
Lane 7 : U2OS cell lysate
Lane 8 : K562 cell lysate
Lane 9 : monkey COS7 cell lysate
Lane 10 : mouse MEF cell lysate
Lane 11 : mouse C2C12 cell lysate
Lane 12 : rat NRK cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : goat anti-mouse-HRP at 1/20000 dilution
Predicted band size: 21 kDa
Immunohistochemical analysis of deparaffinized Human stomach cancer tissue, labeling GLO1 with ab171121 at 1/800 dilution. Colorimetric detection was performed using metal enhanced DAB and tissues counterstained with hematoxylin.
Immunohistochemical analysis of deparaffinized Human prostate cancer tissue, labeling GLO1 with ab171121 at 1/800 dilution. Colorimetric detection was performed using metal enhanced DAB and tissues counterstained with hematoxylin.
ab171121 has not yet been referenced specifically in any publications.