Anti-GLO2 antibody (ab154108)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-GLO2 antibody
See all GLO2 primary antibodies -
Description
Rabbit polyclonal to GLO2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow
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Immunogen
Recombinant fragment corresponding to Human GLO2 aa 84-308.
Database link: Q16775 -
Positive control
- HepG2 and Mouse liver whole cell lysates; Human NCI-N87 xenograft tissue.
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General notes
This product was previously labelled as HAGH
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab154108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 29 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
Hydroxyacylglutathione hydrolase (HAGH) is a thiolesterase which hydrolyses S-lactoyl-glutathione to reduced glutathione and D-lactate. -
Cellular localization
Cytoplasmic and Mitochondrial -
Database links
- Entrez Gene: 509274 Cow
- Entrez Gene: 3029 Human
- Entrez Gene: 14651 Mouse
- Entrez Gene: 24439 Rat
- Omim: 138760 Human
- SwissProt: Q3B7M2 Cow
- SwissProt: Q16775 Human
- SwissProt: Q99KB8 Mouse
see all -
Alternative names
- GLO2 antibody
- GLX II antibody
- Glyoxalase II antibody
see all
Images
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Western blot - Anti-GLO2 antibody (ab154108)Anti-GLO2 antibody (ab154108) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 29 kDa
12% SDS PAGE -
Western blot - Anti-GLO2 antibody (ab154108)Anti-GLO2 antibody (ab154108) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 29 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLO2 antibody (ab154108)Immunohistochemical analysis of paraffin-embedded Human NCI-N87 xenograft tissue labeling GLO2 with ab154108 at 1/100 dilution.
References
This product has been referenced in:
- Di Emidio G et al. SIRT1 participates in the response to methylglyoxal-dependent glycative stress in mouse oocytes and ovary. Biochim Biophys Acta Mol Basis Dis N/A:N/A (2019). WB ; Mouse . Read more (PubMed: 30771486) »
- Cordone V et al. Antiglycative Activity and RAGE Expression in Rett Syndrome. Cells 8:N/A (2019). Read more (PubMed: 30781346) »
