Key features and details
- Rabbit polyclonal to GLO2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-GLO2 antibody
See all GLO2 primary antibodies
DescriptionRabbit polyclonal to GLO2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
Recombinant fragment corresponding to Human GLO2 aa 84-308.
Database link: Q16775
- HepG2 and Mouse liver whole cell lysates; Human NCI-N87 xenograft tissue.
This product was previously labelled as HAGH
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 29 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceHydroxyacylglutathione hydrolase (HAGH) is a thiolesterase which hydrolyses S-lactoyl-glutathione to reduced glutathione and D-lactate.
Cellular localizationCytoplasmic and Mitochondrial
- GLO2 antibody
- GLX II antibody
- Glyoxalase II antibody
Anti-GLO2 antibody (ab154108) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 29 kDa
12% SDS PAGE
Anti-GLO2 antibody (ab154108) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 29 kDa
10% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Human NCI-N87 xenograft tissue labeling GLO2 with ab154108 at 1/100 dilution.
ab154108 has been referenced in 3 publications.
- Emidio GD et al. Methylglyoxal-Dependent Glycative Stress and Deregulation of SIRT1 Functional Network in the Ovary of PCOS Mice. Cells 9:N/A (2020). PubMed: 31947651
- Di Emidio G et al. SIRT1 participates in the response to methylglyoxal-dependent glycative stress in mouse oocytes and ovary. Biochim Biophys Acta Mol Basis Dis N/A:N/A (2019). WB ; Mouse . PubMed: 30771486
- Cordone V et al. Antiglycative Activity and RAGE Expression in Rett Syndrome. Cells 8:N/A (2019). PubMed: 30781346