Overview

  • Product name

    Anti-GLP1 antibody [03] (Biotin)
    See all GLP1 primary antibodies
  • Description

    Mouse monoclonal [03] to GLP1 (Biotin)
  • Host species

    Mouse
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: ELISAmore details
  • Immunogen

    Synthetic peptide:

    EGTFTSNVSSYLEGQAAKEFIAWLVKGRG

    , corresponding to amino acids 9-37 of GLP1 adsorbed onto aluminum hydroxide gel.

  • Epitope

    C terminus of GLP1(1-37), GLP1(7-37) and GLP1(9-37).
  • General notes

    Fusion partner: SP2mIL6 Immunization: Female NMRI x BALB/c mice immunized by intraperitoneal injection.

Properties

Applications

Our Abpromise guarantee covers the use of ab121080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ab121080 is suitable as a capture antibody, or as a biotinylated detection antibody. The dilution guideline is based on use as detection antibody for antigen coated at 1 µg/ml.

Target

  • Function

    Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.
    GLP-1 is a potent stimulator of glucose-dependent insulin release. Play important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Have growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferaton. Inhibits beta cell apoptosis.
    GLP-2 stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability.
    Oxyntomodulin significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness.
    Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
  • Tissue specificity

    Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain.
  • Sequence similarities

    Belongs to the glucagon family.
  • Post-translational
    modifications

    Proglucagon is post-translationally processed in a tissue-specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • GCG antibody
    • Glicentin related polypeptide antibody
    • glicentin-related polypeptide antibody
    • GLP-1 antibody
    • GLP-1(7-36) antibody
    • GLP-1(7-37) antibody
    • GLP-2 antibody
    • GLP1 antibody
    • GLP1, included antibody
    • GLP2 antibody
    • GLP2, included antibody
    • GLUC_HUMAN antibody
    • Glucagon antibody
    • Glucagon like peptide 1 antibody
    • glucagon-like peptide 1 antibody
    • Glucagon-like peptide 1, included antibody
    • Glucagon-like peptide 2 antibody
    • Glucagon-like peptide 2, included antibody
    • GRPP antibody
    • OXM antibody
    • OXY antibody
    • preproglucagon antibody
    see all

References

ab121080 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us.
I have had a look at the products that we have available in our catalogue and I recommend using the following validated antibody pairs for sandwich ELISA (sELISA):
1. Glucagon Like Peptide-1 (active)
I have found two different antibody pairs that have been validated for use in sELISA:
Capture: ab23472 (www.abcam.com/ab23472) and Detection: ab121080(www.abcam.com/ab121080).
or
Capture: ab26278 (www.abcam.com/ab26278) and Detection: ab121072(www.abcam.com/ab121072).
ab26278 is specific for the amidated C terminus of the peptide and does not react with GLP 1 (7-37). It reacts with the amidated C-terminus of GLP 1 (7-36) amide, GLP-1(9-36) and GLP 1 (1-36) amide.
2. Peptide YY
Capture: 121069 (www.abcam.com/ab121069) and Detection: ab121070(www.abcam.com/ab121070).
3. Human Ghrelin
Capture: 121066 (www.abcam.com/ab121066) and Detection: ab121067(www.abcam.com/ab121067).
Attached is a word document with the 4 “standard curves” from the 4 assays. Please note however that these assays have not been optimized and it is very likely that with optimization, you will be able to retrieve a better assay performance than what has been shown here.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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