Overview

  • Product name
    Anti-GLP1 antibody [4F3]
    See all GLP1 primary antibodies
  • Description
    Mouse monoclonal [4F3] to GLP1
  • Host species
    Mouse
  • Specificity
    Reacts with all forms of GLP1, including precursor and GLP1(9-37) /GLP1(9-36amide) metabolite. ab23472 cross-reacts with Liraglutide.
  • Tested applications
    Suitable for: WB, ICC/IF, Sandwich ELISA, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human, Recombinant fragment
    Predicted to work with: Mammals
  • Immunogen

    Full length protein corresponding to GLP1. Synthetic GLP-1(7-36)amide coupled to PPD
    Database link: 2641

  • Epitope
    Mid molecular epitope of GLP1.

Properties

Applications

Our Abpromise guarantee covers the use of ab23472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 4 kDa.
ICC/IF 1/400.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [8G9] to GLP1 (amidated) (ab26278).

We recommend that ab26278 is used as capture antibody. ab26278 binds to the c-terminus and presents the mid-molecular epitope to the ab23472 detection antibody, so it has easy access to it. For the same reason we do not recommend swapping the pair, as the binding of the mid molecular epitope to the coat will not present the c-terminus epitope to the ab26278 antibody in a very favourable manner.

IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • Function
    Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.
    GLP-1 is a potent stimulator of glucose-dependent insulin release. Play important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Have growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferaton. Inhibits beta cell apoptosis.
    GLP-2 stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability.
    Oxyntomodulin significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness.
    Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
  • Tissue specificity
    Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain.
  • Sequence similarities
    Belongs to the glucagon family.
  • Post-translational
    modifications
    Proglucagon is post-translationally processed in a tissue-specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • GCG antibody
    • glicentin-related polypeptide antibody
    • GLP-1 antibody
    • GLP-1(7-36) antibody
    • GLP-1(7-37) antibody
    • GLP-2 antibody
    • GLP1 antibody
    • GLP1, included antibody
    • GLP2 antibody
    • GLP2, included antibody
    • GLUC_HUMAN antibody
    • Glucagon antibody
    • Glucagon like peptide 1 antibody
    • glucagon-like peptide 1 antibody
    • Glucagon-like peptide 1, included antibody
    • Glucagon-like peptide 2 antibody
    • Glucagon-like peptide 2, included antibody
    • GRPP antibody
    • OXM antibody
    • OXY antibody
    • preproglucagon antibody
    see all

Images

  • ab23472 staining GLP1 in Mouse GLUTag intestinal L cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 for 10 minutes and blocked with 5% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/400 in 5% serum) for 24 hours at 4°C. An Alexa Fluor® 555-conjugated Goat anti-mouse IgG polyclonal (1/150) was used as the secondary antibody.

    See Abreview

  • ab23472 (1µg/ml) staining GLP1 in human pancreas, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References

This product has been referenced in:
  • Weng S  et al. Prevalence and factors associated with nonalcoholic fatty pancreas disease and its severity in China. Medicine (Baltimore) 97:e11293 (2018). Read more (PubMed: 29953011) »
  • Xu F  et al. Modified human glucagon-like peptide-1 (GLP-1) produced in E. coli has a long-acting therapeutic effect in type 2 diabetic mice. PLoS One 12:e0181939 (2017). Read more (PubMed: 28750064) »
See all 6 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (ThermoScientific 4-20% precise tris-glycine)
Sample
Rat Tissue lysate - nuclear (Nucleus of the solitary tract and ileum)
Specification
Nucleus of the solitary tract and ileum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Mar 18 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Sample
Mouse Cell (GLUTag intestinal L cell)
Specification
GLUTag intestinal L cell
Permeabilization
Yes - 0.1% Triton X 100, 10 mins
Fixative
Paraformaldehyde

Jeffrey Gagnon

Verified customer

Submitted Sep 13 2013

Answer

Gracias por contactarnos.

Llevando a cabo una búsqueda de anticuerpos contra las proteínas indicadas puedo sugerirte las siguientes alternativas:

Contra CD26: https://www.abcam.com/cd26-antibody-ab28340.html o https://www.abcam.com/cd26-antibody-11d7-ab114033.html. Ambos están testados, y por tanto garantizados en tejidos / células humanas mediante IHC-P y WB.

Para GLP1 habría una opción: https://www.abcam.com/glp1-antibody-4f3-ab23472.html que cumple con los requisitos que buscáis.

Te informo además que actualmente tenemos una promoción por la que al comprar cualquier anticuerpo primario os regalamos otro primario Rabbit Monoclonal. Simplemente indica el código RABMAB-XBSMG en el próximo pedido. Para más información picha https://www.abcam.com/index.html?pageconfig=resource&rid=15447.

Confío en haberte ayudado. En caso contrario no dudes en volvernos a contactar.

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Answer

Thank you for contacting us.
According to our records, an email was sent out to you on Wednesday 7th Nov at 14:53 regarding this question. I am very sorry that you did not receieve this. We have had a few cases where customers have contacted us and our responses were sent straight to their junk inbox which could explain why you didn't receieve our email.
I have copied the answer we sent you below. Please could you reply confirming that you have received this email?

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Answer

Thank you for contacting us.
I have had a look at the products that we have available in our catalogue and I recommend using the following validated antibody pairs for sandwich ELISA (sELISA):
1. Glucagon Like Peptide-1 (active)
I have found two different antibody pairs that have been validated for use in sELISA:
Capture: ab23472 (www.abcam.com/ab23472) and Detection: ab121080(www.abcam.com/ab121080).
or
Capture: ab26278 (www.abcam.com/ab26278) and Detection: ab121072(www.abcam.com/ab121072).
ab26278 is specific for the amidated C terminus of the peptide and does not react with GLP 1 (7-37). It reacts with the amidated C-terminus of GLP 1 (7-36) amide, GLP-1(9-36) and GLP 1 (1-36) amide.
2. Peptide YY
Capture: 121069 (www.abcam.com/ab121069) and Detection: ab121070(www.abcam.com/ab121070).
3. Human Ghrelin
Capture: 121066 (www.abcam.com/ab121066) and Detection: ab121067(www.abcam.com/ab121067).
Attached is a word document with the 4 “standard curves” from the 4 assays. Please note however that these assays have not been optimized and it is very likely that with optimization, you will be able to retrieve a better assay performance than what has been shown here.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question
Answer

Thank you for your call today and for letting us know about the trouble with this antibody.

As we discussed, I'm sending a free of charge vial of this antibody on the order ***, which should arrive on Friday.

Please keep me updated about the results with this antibody, and let me know if there is anything else that we can do for you. Have a great week!

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Answer

Thank you for your inquiry. The peptide used as immunogen is corresponding to amino acids 7 to 36 of the human GLP1 as stated on the datasheet. The exact epitope this antibody was not further mapped and therefore not known. I hope this information is helpful and wish you good luck for your publication.

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Answer

Thank you for contacting us. Indeed, we do not have an antibody tested for all the applications/species. We can however provide you with a testing discount if you want to test one of these antibodies against an untested species or application. In regards to know which one to chose, it depends also on your priorities. My comments on different anti GLP-1 antibodies are: -ab23468 - tested in rat, in ELISA, ICC and WB - As this antibody has been tested in applications with denatured epitope (WB), as well as native epitope (ELISA) I think has reasonable chances to provide good signal in IHC-P as well. Click here (or use the following: https://www.abcam.com/index.html?datasheet=23468). -ab23472 - is indeed tested in WB and IHC-P. The alignment of the immunogen with the rat protein is however not very good with 80% identity. As there is though a stretch of identical amino acids, it might work. Please see here the alignment. http://www.ebi.ac.uk/Tools/services/web/toolresult.ebi?jobId=clustalw2-I20110914-131516-0253-48328932-pg&tool=clustalw2&analysis=alignments -ab26278 - indeed this antibody will react with the rat protein in IHC. However it has not been tested in Western blot and as the epitope is linear in Western blot, it is not possible for me to make a prediction whether this antibody might work in Western blot. It would be necessary to assess this experimentally. I would therefore suggest the best option would be to test ab23468 in IHC-P. I can offer a discount off a future purchase if you buy ab23468 now, test it in IHC-P and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. However if you prefer to test one of the other antibodies in rat or WB respectively, I could also provide you with the same testing offer. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab23468 in IHC-P (or what other antibody you would prefer to test). I will then send a discount code. This code must be issued before purchasing ab23468 so please wait for my reply before ordering. 2. Purchase ab23468 either by phone, fax, or online (www.abcam.com). 3. Test it in IHC-P. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab23468 turns out to be unsuitable for IHC-P, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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