Overview

  • Product name
  • Description
    Rabbit polyclonal to GLP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, IHC-R, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Mammals
  • Immunogen

    Synthetic peptide corresponding to Human GLP1 aa 1-19 conjugated to Bovine Serum Albumin (BSA) (Bis-diazotised tolidine (Bdt)). The mid to C terminal region of GLP 1 [1-19] enabling immunoreactivity with N terminal truncated and C terminally extended forms of GLP 1 (e.g. GLP 1[7 to 36]amide).
    Sequence:

    HDEFERHAEGTFTSDVSSY

  • Positive control
    • Mammalian endocrine pancreas (A cells) and colon (L/EG cells) are heavily labelled with this antiserum.

Applications

Our Abpromise guarantee covers the use of ab22625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-R Use at an assay dependent concentration.
Vibratome slices, All standard crosslinking agents may be used. 1/500 to 1/4000, using overnight incubation and peroxidase anti peroxidase procedure. 1/100 to 1/1000, using overnight incubation and indirect immunofluorescence procedure.
ICC/IF Use at an assay dependent concentration. Used at a dilution of 1/500 for 2 hrs using mouse small intestinal cells (see Abreview for further details).

Target

  • Function
    Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.
    GLP-1 is a potent stimulator of glucose-dependent insulin release. Play important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Have growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferaton. Inhibits beta cell apoptosis.
    GLP-2 stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability.
    Oxyntomodulin significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness.
    Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
  • Tissue specificity
    Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain.
  • Sequence similarities
    Belongs to the glucagon family.
  • Post-translational
    modifications
    Proglucagon is post-translationally processed in a tissue-specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • GCG antibody
    • glicentin-related polypeptide antibody
    • GLP-1 antibody
    • GLP-1(7-36) antibody
    • GLP-1(7-37) antibody
    • GLP-2 antibody
    • GLP1 antibody
    • GLP1, included antibody
    • GLP2 antibody
    • GLP2, included antibody
    • GLUC_HUMAN antibody
    • Glucagon antibody
    • Glucagon like peptide 1 antibody
    • glucagon-like peptide 1 antibody
    • Glucagon-like peptide 1, included antibody
    • Glucagon-like peptide 2 antibody
    • Glucagon-like peptide 2, included antibody
    • GRPP antibody
    • OXM antibody
    • OXY antibody
    • preproglucagon antibody
    see all

Images

  • ab22625 (1/2000) staining GLP1 in human pancreas using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining in the pancreatic islets.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ab22625 at a 1/500 dilution staining mouse small intestine (pancreas) by ICC/IF. The cells were paraformaldehyde fixed and blocked with 5% serum prior to incubation with the antibody for 2 hours. Bound antibody was detected using a Cy2 conjugated donkey anti-rabbit IgG (H+L). As expected, the small intestine staining is in a very low number of entero-endocrine cells (L-cells), laying on the instestinal villi.

    This image is courtesy of an Abreview.

    See Abreview

References

This product has been referenced in:
  • Kuhre RE  et al. Bile acids are important direct and indirect regulators of the secretion of appetite- and metabolism-regulating hormones from the gut and pancreas. Mol Metab 11:84-95 (2018). Read more (PubMed: 29656109) »
  • Beumer J  et al. Enteroendocrine cells switch hormone expression along the crypt-to-villus BMP signalling gradient. Nat Cell Biol 20:909-916 (2018). Read more (PubMed: 30038251) »
See all 10 Publications for this product

Customer reviews and Q&As

1-10 of 15 Abreviews or Q&A

Answer

Thank you for your enquiry.

I can confirm that this particular productis sourced externally, and regrettably they do not have any specific data or information regarding in house testing for cross reactivity with analogue liraglutide (Lys(γ-Glu-palmitoyl)26,Arg34)-GLP-1 (7-37)). We aim to provide as much information as possible to customers, and I am sorry for the inconvenience on this occasion.

The best informationwe have is provided on the datasheet, and there are also the following references for the various forms of GLP-1 which I hope may be helpful to you.

If you have any further questions, please do not hesitate to contact us.


1. Varndell I.M. et al. Localisation of glucagon-like peptide (GLP) immunoreactants in human gut and pancreas using light and electron
microscopic immunocytochemistry. J. Histochem. Cytochem. 33: 1080-1086, 1985.

2. Kreymann B. et al. Characterisation of glucagon-like peptide-1[7-36] amide in the hypothalamus. Brain Res., 502: 325-331, 1989.

3. Kanse S.M. et al. Identification and characterisation of glucagon-like peptide-1[7-36]amide binding sites in the rat brain and lung. FEBS Lett.,
241: 209-212, 1988.

4. Alvarez, E. et al. Expression of the glucagon-like peptide-1 receptor gene in rat brain. J. Neurochem., 66: 920-927, 1996.

5. Oka, J.I., Goto, N. and Kameyama, T. Glucagon-like peptide-1 modulates neuronal activity in the rat’s hippocampus. Neuroreport, 10: 1643-
1646, 1999.

6. Tateishi K. et al. Glucagon-like peptide-1 (GLP-1) molecular forms in human pancreatic endocrine tumours resemble those in intestine rather
than pancreas. Diabetes Res. Clin. Pract., 25: 43-49, 1994.

7. Drucker, D.J. Glucagon-like peptides. Diabetes 47: 159-169, 1998.

8. Naslund, E. et al. GLP-1 slows solid gastric emptying and inhibits insulin, glucagon, and PYY release in humans. Am. J. Physiol., 277: R910-
R916, 1999.

9. Gutzwiller, J.-P. et al. Glucagon-like peptide-1 promotes satiety and reduces food intake in patients with diabetes mellitus type 2. Am. J.
Physiol., 276: R1541-R1544, 1999. [GA1178 citation]

10. Elahi D. et al. The insulinotropic actions of glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1[7-37] in normal and
diabetic subjects. Regul. Peptides, 51: 63-74, 1994.

11. Fehmann H.C. et al. Glucagon-like peptide-1. Eine therapeutische alternative bei diabetes mellitus? Deutsche Med. Wochensch., 119: 275-
279, 1994.

12. Turton M.D. et al. A role for glucagon-like peptide-1 (GLP-1) in the central regulation of feeding. Nature, 379: 69-72, 1996.

13. Goke R. et al. Exendin-4 is a high potency agonist and truncated exendin[9-39]amide is an antagonist at the glucagon-like peptide-1[7-
36]amide receptor of insulin-secreting beta-cells. J. Biol. Chem., 268: 19650-19655, 1993.

14. Van Dijk G. et al. Glucagon-like peptide-1 and satiety. Nature, 385: 214, 1997.

15. Neary, N.M. et al. Peptide YY3-36 and glucagon-like peptide-17-36 inhibit food intake additively. Endocrinology, Sep 8 2005 (epub).

16. O'Shea D. et al. A role for central glucagon-like peptide-1 in temperature regulation. NeuroReport, 7: 830-832, 1996.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Intestine)
Specification
Intestine
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% triton x-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Mar 26 2012

Answer

Thank you for contacting us.

ab26278 would work with Mouse tissue and in IHC-Fr. Please be advised that this is a primary antibody not the secondary antibody. The compatible FITC conjugated secondary antibodies are

ab97239, ab98692 and ab115588.

"Cat # 1072-1002" this is not an Abcam catalogue number so please recheck it.

For mouse on mouse staining, please follow the guidelines given inbelow link;

https://www.abcam.com/ps/pdf/protocols/mouse_on_mouse.pdf

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for your enquiry.

I am sorry I am not able to find catalog number 1072-1002 on our system. Our product numbers always start with ab. We do have the following goat anti mouse IgG FITC conjugated secondary antibody, tested in IHC-Fr, which should be suitable for use with ab26278.

ab7064Goat anti-Mouse IgG H&L (FITC) secondary antibody
FITC conjugated
Flow Cyt, IHC-P, ELISA, IM, ICC/IF, IHC-Fr
https://www.abcam.com/index.html?datasheet=7064 (or use the following: https://www.abcam.com/index.html?datasheet=7064).

I would suggest to bear in mind that the excitation and emission spectra for FITC are very similar to Alexa 488, and so I would not advise using these two conjugates together in duel staining as you will not be able to distinguish between them.

If you would prefer to use a suitable secondary antibody with a different fluorophore, then I can suggest considering the following which has been tested in IHC-Fr:

ab7058Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (TRITC ), pre-adsorbed
TRITC conjugated
https://www.abcam.com/index.html?datasheet=7058 (or use the following: https://www.abcam.com/index.html?datasheet=7058).

I can recommend to review the flourophores you wish to use, and if you require any further help please let me know.

This particular secondary (ab7058) is also cross absorbed, which means it is less likely to cross react. However, as you mention, there is always the possibility of crossreactivity when an anti-mouse secondary is used on mouse tissue. I can recommend to review the following page from our website which provides some useful tips:

https://www.abcam.com/index.html?pageconfig=resource&rid=11465

I hope this will be helpful to you. If you have any further questions, please do not hesitate tocontact us.

Read More

Answer

Thats great news. I have generated a discount code for you:

DISCOUNT CODE: xxxxx
Expiration date: 2012-06-19

As discussed this code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for optimal projection tomography (we don't currently have this as an option on the system but if you choose "whole mount" instead please)and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site:

https://www.abcam.com/Abreviews

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests whatever they may be. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

The terms and conditions applicable to this offer can be found here:

https://www.abcam.com/collaborationdiscount

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If I can be of any help in optimising your experiments once you get started please do let me know. I look forward to hearing how you get on.

Until then,I wish you all the best with your research.

Read More

Answer

Thank you for that extra information.

Having gone through the protocol and the additional information about the technique you have provided, I would suggest trying anti-GLP1 antibody, https://www.abcam.com/GLP1-antibody-ab22625.html. This is a rabbit polyclonal to GLP1 which has been used to detect both mouse and human GLP1 protein.I think it is the most likely to work out of the antibodies we have against thistarget as it has been used successfully in immunohistochemistry with frozen sections, paraffin embedded sections and in immunocytochemistry.

As discussed over the phone, I am able to offer you a testing discount for this(or any other anti-GLP1 antibody which you prefer). This would involve you purchasing the antibody, testing it with optimal projection tomography and sharing the results with us through an abreview. Whether the results are positive or negative, you would then be entitled to a free primary antibody of your choice from our catalogue. More information on this scheme can be found from the following link:

https://www.abcam.com/collaboratordiscount

The only stipulation is that you submit and use the code within 4 months of purchase of the initial antibody.

If you would like to participate in this scheme please do let me know and I will issue you with the discount code.

I look forward to receiving your reply.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Specification
Pancreas
Fixative
10% Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 100%

Abcam user community

Verified customer

Submitted Sep 21 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Duodenum)
Specification
Duodenum
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Feb 03 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Small Intestine)
Specification
Small Intestine
Fixative
Paraformaldehyde
Permeabilization
Yes - TBST
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jan 11 2011

Answer

See the UniProt page for GLP1 molecular processing: http://www.uniprot.org/uniprot/P01275 Different peptides are cleaved from Glucagon, including: Glicentin Oxyntomodulin Glucagon Glucagon-like peptide 1 Glucagon-like peptide 1 (7-37) Glucagon-like peptide 1 (7-36) Glucagon-like peptide 2 The immunogen was from 92 to 110 aminoacids, enabling the antibody to recognize: Glucagon-like peptide 1- cleaved sequence 92 – 128aa Glucagon-like peptide 1 (7-37)- cleaved sequence 98 – 128aa Glucagon-like peptide 1 (7-36)- cleaved sequence 98 – 127

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1-10 of 15 Abreviews or Q&A

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