RecombinantRabMAb

Recombinant Anti-GLP1 antibody [EPR4042-1] (ab108443)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1 antibody [EPR4042-1] (ab108443)
  • ELISA - Anti-GLP1 antibody [EPR4042-1] (ab108443)
  • Immunocytochemistry/ Immunofluorescence - Anti-GLP1 antibody [EPR4042-1] (ab108443)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1 antibody [EPR4042-1] (ab108443)
  • Western blot - Anti-GLP1 antibody [EPR4042-1] (ab108443)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1 antibody [EPR4042-1] (ab108443)
  • ELISA - Anti-GLP1 antibody [EPR4042-1] (ab108443)
  • ELISA - Anti-GLP1 antibody [EPR4042-1] (ab108443)
  • Western blot - Anti-GLP1 antibody [EPR4042-1] (ab108443)
  • Anti-GLP1 antibody [EPR4042-1] (ab108443)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4042-1] to GLP1
  • Suitable for: ELISA, ICC/IF, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-GLP1 antibody [EPR4042-1]
    See all GLP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4042-1] to GLP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ELISA, ICC/IF, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human GLP1 aa 50-150. The exact sequence is proprietary.
    Database link: P01275

  • Positive control

    • Glicentin polypeptide, Human pancreas tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Applications

Our Abpromise guarantee covers the use of ab108443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 - 1 µg/ml.
ICC/IF 1/800.
WB 1/2000. Predicted molecular weight: 21 kDa.

For unpurified, use 1/200.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.
    GLP-1 is a potent stimulator of glucose-dependent insulin release. Play important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Have growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferaton. Inhibits beta cell apoptosis.
    GLP-2 stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability.
    Oxyntomodulin significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness.
    Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.

  • Tissue specificity

    Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain.

  • Sequence similarities

    Belongs to the glucagon family.

  • Post-translational
    modifications

    Proglucagon is post-translationally processed in a tissue-specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • GCG antibody
      • Glicentin related polypeptide antibody
      • glicentin-related polypeptide antibody
      • GLP-1 antibody
      • GLP-1(7-36) antibody
      • GLP-1(7-37) antibody
      • GLP-2 antibody
      • GLP1 antibody
      • GLP1, included antibody
      • GLP2 antibody
      • GLP2, included antibody
      • GLUC_HUMAN antibody
      • Glucagon antibody
      • Glucagon like peptide 1 antibody
      • glucagon-like peptide 1 antibody
      • Glucagon-like peptide 1, included antibody
      • Glucagon-like peptide 2 antibody
      • Glucagon-like peptide 2, included antibody
      • GRPP antibody
      • OXM antibody
      • OXY antibody
      • preproglucagon antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1 antibody [EPR4042-1] (ab108443)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1 antibody [EPR4042-1] (ab108443)

      Unpurified ab108443, at 1/100 dilution, staining Glicentin in Human pancreas tissue by immunohistochemistry

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ELISA - Anti-GLP1 antibody [EPR4042-1] (ab108443)
      ELISA - Anti-GLP1 antibody [EPR4042-1] (ab108443)

      1 ug/mL of Glucagon-like Peptide (GLP-1) was coated into 96-wells. Various concentrations of anti-Glucagon-Like Peptide 1 (GLP-1, 7-36,amide) rabbit monoclonal antibody (unpurified ab108443) was added. HRP conjugated goat anti-rabbit IgG antibody was used to develop the color.

    • Immunocytochemistry/ Immunofluorescence - Anti-GLP1 antibody [EPR4042-1] (ab108443)
      Immunocytochemistry/ Immunofluorescence - Anti-GLP1 antibody [EPR4042-1] (ab108443)
      Immunofluorescence staining of BxPC-3 cells with purified ab108443 at a working dilution of 1/800, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab108443 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1 antibody [EPR4042-1] (ab108443)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1 antibody [EPR4042-1] (ab108443)
      Immunohistochemical staining of paraffin embedded human pancreas with purified ab108443 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Western blot - Anti-GLP1 antibody [EPR4042-1] (ab108443)
      Western blot - Anti-GLP1 antibody [EPR4042-1] (ab108443)
      Anti-GLP1 antibody [EPR4042-1] (ab108443) at 1/2000 dilution (purified) + human fetal pancreas lysate at 10 µg

      Secondary
      HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 21 kDa
      Observed band size: 11 kDa
      why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1 antibody [EPR4042-1] (ab108443)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1 antibody [EPR4042-1] (ab108443)
      Immunohistochemical staining of paraffin embedded human liver with purified ab108443 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • ELISA - Anti-GLP1 antibody [EPR4042-1] (ab108443)
      ELISA - Anti-GLP1 antibody [EPR4042-1] (ab108443)

      Antigen dose-response curve using purified ab108443. Antibody concentration of 1 µg/mL. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

    • ELISA - Anti-GLP1 antibody [EPR4042-1] (ab108443)
      ELISA - Anti-GLP1 antibody [EPR4042-1] (ab108443)

      Antibody dose-response curve using purified ab108443. Antigen concentration of 1 µg/mL. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

    • Western blot - Anti-GLP1 antibody [EPR4042-1] (ab108443)
      Western blot - Anti-GLP1 antibody [EPR4042-1] (ab108443)
      Anti-GLP1 antibody [EPR4042-1] (ab108443) at 1/200 dilution (unpurified) + Glicentin polypeptide at 0.005 µg

      Predicted band size: 21 kDa

    • Anti-GLP1 antibody [EPR4042-1] (ab108443)
      Anti-GLP1 antibody [EPR4042-1] (ab108443)

    References (0)

    Publishing research using ab108443? Please let us know so that we can cite the reference in this datasheet.

    ab108443 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Submit a question

    Question

    Will this antibody cross react with glucagon, proglucagon or GLP-2?

    Read More
    Answer

    ab108443 is predicted to recognize proglucagon, but not glucagon or GLP-2, based on sequence analysis.

    Read More

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