Overview

  • Product name
  • Description
    Goat polyclonal to GLP1R
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human GLP1R aa 211-222 (internal sequence) (Cysteine residue). The immunizing peptide represents part of an extracellular region (NP_002053.3).
    Sequence:

    QHQWDGLLSYQD


    Database link: P43220

  • Positive control
    • Human Brain (Cerebral and Frontal Cortex, Hippocampus) lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab186051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa).

Target

Images

  • Anti-GLP1R antibody (ab186051) at 1 µg/ml + Human hippocampus lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 53 kDa
    Observed band size: 55 kDa (why is the actual band size different from the predicted?)



    Primary incubation was 1 hour.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (small cell lung cancer cell line 16T)
Permeabilization
No
Gating Strategy
single cells with FSC and SSC, live cells with low DAPI, positive/negative cells
Specification
small cell lung cancer cell line 16T
Preparation
Cell harvesting/tissue preparation method: Cell harvesting: cells grow as floating spheres. Collect by spinning, dissociate spheres with TrypLE, single cell through 40um cell strainer
Sample buffer: PBS + 10% FBS
Fixation
none
Username

Abcam user community

Verified customer

Submitted Jul 10 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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