Recombinant
RabMAb

Recombinant Anti-GLP1R antibody [EPR21819] - BSA and Azide free (ab236269)

Overview

  • Product name

    Anti-GLP1R antibody [EPR21819] - BSA and Azide free
    See all GLP1R primary antibodies
  • Description

    Rabbit monoclonal [EPR21819] to GLP1R - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Mouse GLP1R aa 1-150. The exact sequence is proprietary.
    Database link: O35659

  • Positive control

    • IHC-P: Mouse pancreas tissue.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®). 

    ab236269 is a PBS-only buffer format of ab218532. Please refer to ab218532 for recommended dilutions, protocols, and image data.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab236269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

WB Use at an assay dependent concentration. Predicted molecular weight: 53 kDa.
IHC-Fr Use at an assay dependent concentration.

Heat-mediated antigen retrieval using Tris-EDTA buffer (pH9.0) (ab94681)

Target

  • Function

    This is a receptor for glucagon-like peptide 1. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 2 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • GLP 1 R antibody
    • GLP 1 receptor antibody
    • GLP 1R antibody
    • GLP antibody
    • GLP-1 receptor antibody
    • GLP-1-R antibody
    • GLP-1R antibody
    • GLP1R antibody
    • GLP1R_HUMAN antibody
    • Glucagon like peptide 1 receptor antibody
    • Glucagon-like peptide 1 receptor antibody
    • MGC138331 antibody
    • OTTHUMP00000016340 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded rat pancreas tissue stained for GLP1R using ab218532 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and membranous staining in rat pancreatic islet cells (PMID: 24467746) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Performed heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218532).

  • 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat pancreas tissue stained for GLP1R (green) using ab218532 at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Positive cytoplasmic and membranous staining in rat pancreatic islet cells (PMID: 18541709; 24695667) is observed. Nuclear counterstained with DAPI.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.

    Heat-mediated antigen retrieval using Tris-EDTA buffer (pH9.0) (ab94681).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218532).

  • 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized mouse pancreas tissue stained for GLP1R (green) using ab218532 at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Positive cytoplasmic and membranous staining in mouse pancreatic islet cells (PMID: 18541709; 24695667) is observed. Nuclear counterstained with DAPI.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.

    Heat-mediated antigen retrieval using Tris-EDTA buffer (pH9.0) (ab94681).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218532).

  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue stained for GLP1R using ab218532 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining of mouse pancreatic islet cells (PMID: 24467746) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Performed heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218532).

     

References

ab236269 has not yet been referenced specifically in any publications.

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