Overview

  • Product name

  • Description

    Goat polyclonal to GluA4
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Human GluA4 aa 280-293 (internal sequence). (NP_000820.3; NP_001070711.1; NP_001070712.1)
    Sequence:

    C-KKLDQREYPGSETP

  • Positive control

    • Human Brain, Cortex; Human Cerebellum lysate
  • General notes

    Previously labelled as Ionotropic Glutamate receptor 4.

Properties

Applications

Our Abpromise guarantee covers the use of ab115322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 101 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
  • Sequence similarities

    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA4 subfamily.
  • Post-translational
    modifications

    Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-611 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
  • Cellular localization

    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AMPA 4 antibody
    • AMPA selective glutamate receptor 4 antibody
    • AMPA-selective glutamate receptor 4 antibody
    • AMPA4 antibody
    • GluA 4 antibody
    • GluA4 antibody
    • GluR 4 antibody
    • GluR D antibody
    • GluR-4 antibody
    • GluR-D antibody
    • GluR4 antibody
    • GLUR4C antibody
    • GLURD antibody
    • Glutamate receptor 4 antibody
    • Glutamate receptor ionotrophic AMPA 4 antibody
    • Glutamate receptor ionotropic antibody
    • GRIA 4 antibody
    • Gria4 antibody
    • GRIA4_HUMAN antibody
    • Ionotropic Glutamate receptor 4 antibody
    see all

Images

  • Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with ab115322 at 5 µg/ml, followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-GluA4 antibody (ab115322) at 0.3 µg/ml (incubated for 1 hour) + Human Cerebellum lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 101 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Rogers EJ  et al. An IQSEC2 Mutation Associated With Intellectual Disability and Autism Results in Decreased Surface AMPA Receptors. Front Mol Neurosci 12:43 (2019). Read more (PubMed: 30842726) »
  • Polepalli JS  et al. Modulation of excitation on parvalbumin interneurons by neuroligin-3 regulates the hippocampal network. Nat Neurosci 20:219-229 (2017). Read more (PubMed: 28067903) »
See all 2 Publications for this product

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