• Product name

  • Description

    Goat polyclonal to GluA4
  • Host species

  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Human GluA4 aa 280-293 (internal sequence). (NP_000820.3; NP_001070711.1; NP_001070712.1)


  • Positive control

    • Human Brain, Cortex; Human Cerebellum lysate
  • General notes

    Previously labelled as Ionotropic Glutamate receptor 4.



Our Abpromise guarantee covers the use of ab115322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 101 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function

    Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
  • Sequence similarities

    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA4 subfamily.
  • Post-translational

    Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-611 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
  • Cellular localization

    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AMPA 4 antibody
    • AMPA selective glutamate receptor 4 antibody
    • AMPA-selective glutamate receptor 4 antibody
    • AMPA4 antibody
    • GluA 4 antibody
    • GluA4 antibody
    • GluR 4 antibody
    • GluR D antibody
    • GluR-4 antibody
    • GluR-D antibody
    • GluR4 antibody
    • GLUR4C antibody
    • GLURD antibody
    • Glutamate receptor 4 antibody
    • Glutamate receptor ionotrophic AMPA 4 antibody
    • Glutamate receptor ionotropic antibody
    • GRIA 4 antibody
    • Gria4 antibody
    • GRIA4_HUMAN antibody
    • Ionotropic Glutamate receptor 4 antibody
    see all


  • Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with ab115322 at 5 µg/ml, followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-GluA4 antibody (ab115322) at 0.3 µg/ml (incubated for 1 hour) + Human Cerebellum lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 101 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?


This product has been referenced in:

  • Rogers EJ  et al. An IQSEC2 Mutation Associated With Intellectual Disability and Autism Results in Decreased Surface AMPA Receptors. Front Mol Neurosci 12:43 (2019). Read more (PubMed: 30842726) »
  • Polepalli JS  et al. Modulation of excitation on parvalbumin interneurons by neuroligin-3 regulates the hippocampal network. Nat Neurosci 20:219-229 (2017). Read more (PubMed: 28067903) »
See all 2 Publications for this product

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