Recombinant Anti-GluA4 antibody [EPR2512(2)] (ab109431)
![Western blot - Anti-GluA4 antibody [EPR2512(2)] (ab109431)](https://www.abcam.com/ps/products/109/ab109431/Images/ab109431-382386-anti-glua4-antibody-epr2512-2-westernblot-brain-rat.jpg "Western blot - Anti-GluA4 antibody [EPR2512(2)] (ab109431)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2512(2)] to GluA4
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-GluA4 antibody [EPR2512(2)]
See all GluA4 primary antibodies -
Description
Rabbit monoclonal [EPR2512(2)] to GluA4 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Rat brain, Mouse brain, Human cerebellum, and SH-SY5Y lysates; SH-SY5Y cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2512(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109431 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 101 kDa.
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| Notes |
|---|
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 101 kDa. |
Target
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Function
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA4 subfamily. -
Post-translational
modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-611 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2893 Human
- Entrez Gene: 14802 Mouse
- Entrez Gene: 29629 Rat
- Omim: 138246 Human
- SwissProt: P48058 Human
- SwissProt: Q9Z2W8 Mouse
- SwissProt: P19493 Rat
- Unigene: 503743 Human
see all -
Alternative names
- AMPA 4 antibody
- AMPA selective glutamate receptor 4 antibody
- AMPA-selective glutamate receptor 4 antibody
see all
Images
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Western blot - Anti-GluA4 antibody [EPR2512(2)] (ab109431)All lanes : Anti-GluA4 antibody [EPR2512(2)] (ab109431) at 1/1000 dilution
Lane 1 : Rat brain prepared in RIPA lysis method
Lane 2 : Rat brain prepared in 1%SDS Hot lysis method
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 101 kDa
Observed band size: 101 kDaWe recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.
Blocking/Diluting Buffer and concentration:
5% NFDM/TBST -
![Western blot - Anti-GluA4 antibody [EPR2512(2)] (ab109431)](https://www.abcam.com/ps/products/109/ab109431/Images/ab109431-122267-anti-glua4-antibody-epr25122-western-blot.jpg)
Western blot - Anti-GluA4 antibody [EPR2512(2)] (ab109431)All lanes : Anti-GluA4 antibody [EPR2512(2)] (ab109431) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Human cerebellum lysate
Lane 4 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 101 kDa -
![Flow Cytometry (Intracellular) - Anti-GluA4 antibody [EPR2512(2)] (ab109431)](https://www.abcam.com/ps/products/109/ab109431/Images/ab109431-122266-anti-glua4-antibody-epr25122-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-GluA4 antibody [EPR2512(2)] (ab109431)Intracellular flow cytometric analysis of permeabilized SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells using ab109431 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
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![Anti-GluA4 antibody [EPR2512(2)] (ab109431)](https://www.abcam.com/ps/products/109/ab109431/Images/ab109431-4-benefits-of-recombinant-antibodies.png)
Anti-GluA4 antibody [EPR2512(2)] (ab109431)
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab109431 has been referenced in 1 publication.
- Borland JM et al. Effect of Aggressive Experience in Female Syrian Hamsters on Glutamate Receptor Expression in the Nucleus Accumbens. Front Behav Neurosci 14:583395 (2020). PubMed: 33328919