RecombinantRabMAb

Recombinant Anti-GluA4 antibody [EPR2512(2)] (ab109431)

Submit a review Submit a question

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2512(2)] to GluA4
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GluA4 antibody [EPR2512(2)]
    See all GluA4 primary antibodies

  • Description

    Rabbit monoclonal [EPR2512(2)] to GluA4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human GluA4 aa 850-950 (intracellular). The exact sequence is proprietary.

  • Positive control

    • Rat brain, Mouse brain, Human cerebellum, and SH-SY5Y lysates; SH-SY5Y cells

  • General notes

     

     

    Previously labelled as Ionotropic Glutamate receptor 4.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab109431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 101 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.

    • Target

    • Function

      Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.

    • Sequence similarities

      Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA4 subfamily.

    • Post-translational
      modifications

      Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-611 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.

    • Cellular localization

      Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • AMPA 4 antibody
      • AMPA selective glutamate receptor 4 antibody
      • AMPA-selective glutamate receptor 4 antibody
      • AMPA4 antibody
      • GluA 4 antibody
      • GluA4 antibody
      • GluR 4 antibody
      • GluR D antibody
      • GluR-4 antibody
      • GluR-D antibody
      • GluR4 antibody
      • GLUR4C antibody
      • GLURD antibody
      • Glutamate receptor 4 antibody
      • Glutamate receptor ionotrophic AMPA 4 antibody
      • Glutamate receptor ionotropic antibody
      • GRIA 4 antibody
      • Gria4 antibody
      • GRIA4_HUMAN antibody
      • Ionotropic Glutamate receptor 4 antibody
      see all

    Images

    • Western blot - Anti-GluA4 antibody [EPR2512(2)] (ab109431)
      Western blot - Anti-GluA4 antibody [EPR2512(2)] (ab109431)
      All lanes : Anti-GluA4 antibody [EPR2512(2)] (ab109431) at 1/1000 dilution

      Lane 1 : Rat brain prepared in RIPA lysis method
      Lane 2 : Rat brain prepared in 1%SDS Hot lysis method

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 101 kDa
      Observed band size: 101 kDa



      We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.

      Blocking/Diluting Buffer and concentration:
      5% NFDM/TBST

    • Western blot - Anti-GluA4 antibody [EPR2512(2)] (ab109431)
      Western blot - Anti-GluA4 antibody [EPR2512(2)] (ab109431)
      All lanes : Anti-GluA4 antibody [EPR2512(2)] (ab109431) at 1/1000 dilution

      Lane 1 : Rat brain lysate
      Lane 2 : Mouse brain lysate
      Lane 3 : Human cerebellum lysate
      Lane 4 : SH-SY5Y lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 101 kDa

    • Flow Cytometry - Anti-GluA4 antibody [EPR2512(2)] (ab109431)
      Flow Cytometry - Anti-GluA4 antibody [EPR2512(2)] (ab109431)

      Flow cytometric analysis of permeabilized SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells using ab109431 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

    • Anti-GluA4 antibody [EPR2512(2)] (ab109431)
      Anti-GluA4 antibody [EPR2512(2)] (ab109431)

    References (0)

    Publishing research using ab109431? Please let us know so that we can cite the reference in this datasheet.

    ab109431 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab109431.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com