Recombinant Anti-GluA4 antibody [EPR2512(2)] (ab109431)
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- References
- Protocols
Overview
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Product name
Anti-GluA4 antibody [EPR2512(2)]
See all GluA4 primary antibodies -
Description
Rabbit monoclonal [EPR2512(2)] to GluA4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human GluA4 aa 850-950 (intracellular). The exact sequence is proprietary.
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Positive control
- Rat brain, Mouse brain, Human cerebellum, and SH-SY5Y lysates; SH-SY5Y cells
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General notes
Previously labelled as Ionotropic Glutamate receptor 4.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2512(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab109431 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 101 kDa. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA4 subfamily. -
Post-translational
modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-611 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2893 Human
- Entrez Gene: 14802 Mouse
- Entrez Gene: 29629 Rat
- Omim: 138246 Human
- SwissProt: P48058 Human
- SwissProt: Q9Z2W8 Mouse
- SwissProt: P19493 Rat
- Unigene: 503743 Human
see all -
Alternative names
- AMPA 4 antibody
- AMPA selective glutamate receptor 4 antibody
- AMPA-selective glutamate receptor 4 antibody
see all
Images
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![Western blot - Anti-GluA4 antibody [EPR2512(2)] (ab109431)](https://www.abcam.com/ps/products/109/ab109431/Images/ab109431-122267-anti-glua4-antibody-epr25122-western-blot.jpg)
Western blot - Anti-GluA4 antibody [EPR2512(2)] (ab109431)All lanes : Anti-GluA4 antibody [EPR2512(2)] (ab109431) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Human cerebellum lysate
Lane 4 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 101 kDa -
![Flow Cytometry - Anti-GluA4 antibody [EPR2512(2)] (ab109431)](https://www.abcam.com/ps/products/109/ab109431/Images/ab109431-122266-anti-glua4-antibody-epr25122-flow-cytometry.jpg)
Flow Cytometry - Anti-GluA4 antibody [EPR2512(2)] (ab109431)Flow cytometric analysis of permeabilized SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells using ab109431 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Datasheets and documents
References
ab109431 has not yet been referenced specifically in any publications.
