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  1. Link

    glua4-antibody-epr25122-ab109431.pdf

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Neuroscience Neurotransmission Receptors / Channels GPCR Glutamate Receptors
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RecombinantRabMAb

Recombinant Anti-GluA4 antibody [EPR2512(2)] (ab109431)

  • Datasheet
  • SDS
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Western blot - Anti-GluA4 antibody [EPR2512(2)] (ab109431)
  • Western blot - Anti-GluA4 antibody [EPR2512(2)] (ab109431)
  • Flow Cytometry (Intracellular) - Anti-GluA4 antibody [EPR2512(2)] (ab109431)
  • Anti-GluA4 antibody [EPR2512(2)] (ab109431)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2512(2)] to GluA4
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Primary
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Anti-PDE4 antibody (ab14628)
Protein
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Recombinant Human GluA4 protein (ab152441)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-GluA4 antibody [EPR2512(2)]
    See all GluA4 primary antibodies
  • Description

    Rabbit monoclonal [EPR2512(2)] to GluA4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Rat brain, Mouse brain, Human cerebellum, and SH-SY5Y lysates; SH-SY5Y cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR2512(2)
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Glutamate Receptors
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-GluA4 antibody [EPR2512(2)] - BSA and Azide free (ab232015)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Rat brain normal tissue lysate - membrane extract (ab29473)
  • Recombinant Protein

    • Recombinant Human GluA4 protein (ab152441)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/10000. Predicted molecular weight: 101 kDa.
Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/10000. Predicted molecular weight: 101 kDa.
Application notes
Is unsuitable for ICC/IF,IHC-P or IP.

Target

  • Function

    Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
  • Sequence similarities

    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA4 subfamily.
  • Post-translational
    modifications

    Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-611 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
  • Cellular localization

    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Target information above from: UniProt accession P48058 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2893 Human
    • Entrez Gene: 14802 Mouse
    • Entrez Gene: 29629 Rat
    • Omim: 138246 Human
    • SwissProt: P48058 Human
    • SwissProt: Q9Z2W8 Mouse
    • SwissProt: P19493 Rat
    • Unigene: 503743 Human
    • Unigene: 209263 Mouse
    • Unigene: 10938 Rat
    see all
  • Alternative names

    • AMPA 4 antibody
    • AMPA selective glutamate receptor 4 antibody
    • AMPA-selective glutamate receptor 4 antibody
    • AMPA4 antibody
    • GluA 4 antibody
    • GluA4 antibody
    • GluR 4 antibody
    • GluR D antibody
    • GluR-4 antibody
    • GluR-D antibody
    • GluR4 antibody
    • GLUR4C antibody
    • GLURD antibody
    • Glutamate receptor 4 antibody
    • Glutamate receptor ionotrophic AMPA 4 antibody
    • Glutamate receptor ionotropic antibody
    • GRIA 4 antibody
    • Gria4 antibody
    • GRIA4_HUMAN antibody
    • Ionotropic Glutamate receptor 4 antibody
    see all

Images

  • Western blot - Anti-GluA4 antibody [EPR2512(2)] (ab109431)
    Western blot - Anti-GluA4 antibody [EPR2512(2)] (ab109431)
    All lanes : Anti-GluA4 antibody [EPR2512(2)] (ab109431) at 1/1000 dilution

    Lane 1 : Rat brain prepared in RIPA lysis method
    Lane 2 : Rat brain prepared in 1%SDS Hot lysis method

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 101 kDa
    Observed band size: 101 kDa



    We recommend to use 1%SDS Hot lysis prepare method to get desired Western Blot results.

    Blocking/Diluting Buffer and concentration:
    5% NFDM/TBST

  • Western blot - Anti-GluA4 antibody [EPR2512(2)] (ab109431)
    Western blot - Anti-GluA4 antibody [EPR2512(2)] (ab109431)
    All lanes : Anti-GluA4 antibody [EPR2512(2)] (ab109431) at 1/1000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Human cerebellum lysate
    Lane 4 : SH-SY5Y lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 101 kDa

  • Flow Cytometry (Intracellular) - Anti-GluA4 antibody [EPR2512(2)] (ab109431)
    Flow Cytometry (Intracellular) - Anti-GluA4 antibody [EPR2512(2)] (ab109431)

    Intracellular flow cytometric analysis of permeabilized SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells using ab109431 at 1/10 dilution (red) or a rabbit IgG (negative) (green). 

     

     

  • Anti-GluA4 antibody [EPR2512(2)] (ab109431)
    Anti-GluA4 antibody [EPR2512(2)] (ab109431)

Protocols

  • Western blot protocols
  • Flow cytometry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab109431? Please let us know so that we can cite the reference in this datasheet.

ab109431 has been referenced in 1 publication.

  • Borland JM  et al. Effect of Aggressive Experience in Female Syrian Hamsters on Glutamate Receptor Expression in the Nucleus Accumbens. Front Behav Neurosci 14:583395 (2020). PubMed: 33328919

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