Recombinant
RabMAb

Recombinant Anti-GluA4 antibody [EPR2512(2)] - BSA and Azide free (ab232015)

Overview

  • Product name

    Anti-GluA4 antibody [EPR2512(2)] - BSA and Azide free
    See all GluA4 primary antibodies
  • Description

    Rabbit monoclonal [EPR2512(2)] to GluA4 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GluA4 aa 850-950 (intracellular). The exact sequence is proprietary.

  • Positive control

    • Flow Cytometry: SH-SY5Y cells.
  • General notes

    ab232015 is the carrier-free version of ab109431 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab232015 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Previously labelled as Ionotropic Glutamate receptor 4.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB Use at an assay dependent concentration. Predicted molecular weight: 101 kDa.

Target

  • Function

    Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
  • Sequence similarities

    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA4 subfamily.
  • Post-translational
    modifications

    Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-611 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
  • Cellular localization

    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AMPA 4 antibody
    • AMPA selective glutamate receptor 4 antibody
    • AMPA-selective glutamate receptor 4 antibody
    • AMPA4 antibody
    • GluA 4 antibody
    • GluA4 antibody
    • GluR 4 antibody
    • GluR D antibody
    • GluR-4 antibody
    • GluR-D antibody
    • GluR4 antibody
    • GLUR4C antibody
    • GLURD antibody
    • Glutamate receptor 4 antibody
    • Glutamate receptor ionotrophic AMPA 4 antibody
    • Glutamate receptor ionotropic antibody
    • GRIA 4 antibody
    • Gria4 antibody
    • GRIA4_HUMAN antibody
    • Ionotropic Glutamate receptor 4 antibody
    see all

Images

  • Flow cytometric analysis of permeabilized SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells using ab109431 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109431).

References

ab232015 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab232015.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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