Anti-GluA4 (phospho S862) antibody (ab61054)
Key features and details
- Rabbit polyclonal to GluA4 (phospho S862)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GluA4 (phospho S862) antibody
See all GluA4 primary antibodies -
Description
Rabbit polyclonal to GluA4 (phospho S862) -
Host species
Rabbit -
Specificity
Detects target only when phosphorylated at serine 862. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human GluA4 (phospho S862).
Sequence:(R-L-SP-I-T)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab61054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa). |
Target
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Function
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA4 subfamily. -
Post-translational
modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-611 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2893 Human
- Entrez Gene: 14802 Mouse
- Omim: 138246 Human
- SwissProt: P48058 Human
- SwissProt: Q9Z2W8 Mouse
- Unigene: 503743 Human
- Unigene: 209263 Mouse
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Alternative names
- AMPA 4 antibody
- AMPA selective glutamate receptor 4 antibody
- AMPA-selective glutamate receptor 4 antibody
see all
Images
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All lanes : Anti-GluA4 (phospho S862) antibody (ab61054) at 1/500 dilution
Lane 1 : extracts from HepG2 cells treated with Forskolin (40nM, 30mins)
Lane 2 : extracts from HepG2 cells treated with Forskolin (40nM, 30mins) with immunizing phosphopeptide
Predicted band size: 102 kDa
Observed band size: 102 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab61054 has not yet been referenced specifically in any publications.