Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Pancreas)
Permeabilization
No
Specification
Pancreas
Blocking step
serum +1% BSA in 0.1% PBST as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% serum +1% BSA in 0.1%PBST
Dilution
1/100
Secondary antibody
Dilution
1/100
Name
Non-Abcam antibody was used: Donkey anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Additional Notes
Glucagon (green), insulin (red), counterstained with DAPI (blue)
Abcam response
Thank you for having tested ab92517 in an untested application (IHC-Fr). We will welcome any further feedback in IHC-Fr. So please contact our Scientific support team to participate to our AbTrial program.
The Terms and Conditions of this offer can be found at www.abcam.com/abtrial.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Mar 24 2017