Overview

  • Product name
    Anti-Glucagon antibody [K79bB10]
    See all Glucagon primary antibodies
  • Description
    Mouse monoclonal [K79bB10] to Glucagon
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, ICC/IF, IHC-Fr, RIA, Dot blotmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human, Pig, Common marmoset
    Predicted to work with: Rabbit, Guinea pig, Cat, Syrian hamster
  • Immunogen

    Polymerized porcine glucagon.

  • Positive control
    • Pancreatic glucagon for RIA.
  • General notes

    Production of this antibody has been changed on 8th July 2016. The following lots are from ascites and are still in stock as of 8th July 2016: GR260160 and GR268340.  Lot numbers higher than GR268340 will be purified from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab10988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 21821034
RIA Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.

Target

Images

  • ab10988 at 1/200 staining mouse pancreas tissue sections by IHC-P. The tissue sections were paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 16 hours at 4°C. A Texas Red conjugated donkey anti-mouse antibody was used as the secondary.

    See Abreview

  • Immunohistochemical analysis of Mouse pancreas tissue sections labeling Glucagon with ab10988 at 1/1000 dilution

    See Abreview

  • Immunohistochemical analysis of Mouse pancreas tissue labeling Glucagon with ab10988 at 1/1000 dilution.

    See Abreview

  • ab10988 staining Glucagon in rat pancreatic tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in paraformaldehyde, blocked with 2% serum for 1 hour at 25°C, then incubated with ab10988 at a 1/200 dilution for 1 hour at 25°C. The secondary used was a donkey anti-mouse IgG (H+L) conjugated to Texas Red, used at a 1/250 dilution. Nuclei counterstained with DAPI, green is staining for insulin.

    See Abreview

  • ab10988 staining human islets (beta cells) by ICC/IF.  Cells were PFA fixed and blocked in 2% serum for 1 hour at 25°C prior to incubating with ab10988 for 1 hour at 25°C.  A Cy3® conjugated goat anti-mouse antibody diluted 1/500 was used as the secondary.

    See Abreview

References

This product has been referenced in:
  • Wang G  et al. Human amniotic epithelial cells regulate osteoblast differentiation through the secretion of TGFß1 and microRNA-34a-5p. Int J Mol Med 41:791-799 (2018). ICC/IF ; Human . Read more (PubMed: 29207015) »
  • Sheets TP  et al. Targeted Mutation of NGN3 Gene Disrupts Pancreatic Endocrine Cell Development in Pigs. Sci Rep 8:3582 (2018). Read more (PubMed: 29483633) »
See all 56 Publications for this product

Customer reviews and Q&As

1-10 of 17 Abreviews or Q&A

Answer

Thank you for contacting us.

For ab10988: ICC/IF was added based on published references and a customer Abreview. Thus, a protocol would be based on these sources. We have not tested it ourselves in this application.

The abreview information can be found here. This is all the information we have:
https://www.abcam.com/index.html?datasheet=10988&tab=abreviews&intabreviewid=10554

And the references are as follows:

1) Lu J et al. Alpha cell-specific Men1 ablation triggers the transdifferentiation of glucagon-expressing cells and insulinoma development. Gastroenterology 138:1954-65 (2010). ICC/IF, IHC-P; Mouse. PubMed: 20138042

2) Hanley S & Rosenberg L Transforming growth factor beta is a critical regulator of adult human islet plasticity. Mol Endocrinol 21:1467-77 (2007). ICC/IF; Human. PubMed: 17405902

I have not yet heard back yet from the lab regading the other antibodies, but will let you know as soon asI do.

Thank you for your patience.

I hope this information is of some help so far. In the meantime, please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
No
Specification
Pancreas
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% Formalin

Miss. Barbara Makela

Verified customer

Submitted Jun 22 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (pancreas)
Antigen retrieval step
None
Permeabilization
No
Specification
pancreas
Blocking step
dako antibody diluent with background reducing components as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jan 03 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Syrian Hamster Tissue sections (pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Permeabilization
No
Specification
pancreas
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted May 29 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Permeabilization
No
Specification
pancreas
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted May 28 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (pancreas)
Permeabilization
No
Specification
pancreas
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted May 28 2015

Answer

Thank you for contacting us and your interest in our products. I am sorry for the delay in getting back to you in regards to the Anti-Glucagon antibody [K79bB10] (ab10988).

Glucagon is cleaved into 8 peptide chains one of them is GLP1. As this anhtibody was raised against the polymerised porcine glucagon I cannot say wether the anitbody will detect the GLP1 portion of the protein in particular. I would suggest if you are interested in detecting the GLP1 protein, you may want to consider the following antibodies directed against GLP1:

https://www.abcam.com/Search?Keywords=GLP1&fReset=1&pt=-1&source=TopSearch&btnSearch=Search#Search?AppliedFacets.FacetProductType=Primary+antibodies&AppliedFacets.FacetTargetName=GLP1&IsFacetsOnly=False&PageNumber=1&Keywords%255B0%255D=GLP1&X-Requested-With=XMLHttpRequest

I hope this information has been helpful. If you have any further questions, please do not hesitate to let me know.

Read More

Answer

Thank you for contacting us.

I have contacted the lab regarding the 4 antibodies you had inquired about, and will be in touch with more informaton as soon as I have it.

Thank you for your patience!


Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Question
Answer

Thank you for contacting us. I was able to find a paper describing production and characterization of the ab10988 clone, K79bB10, but as far as I can tell, the epitope has not been mapped. Here is a link to the paper's abstract: http://www.ncbi.nlm.nih.gov/pubmed/3138717 Production and use of monoclonal glucagon and insulin antibodies--reduction of pancreatic insulin in rats by treatment with complete Freund's adjuvant. Acta Histochem Suppl. 1988;35:217-23. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreas)
Specification
pancreas
Fixative
Formaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
(agent) for 10 minute(s) · Concentration: 100% · Temperature: RT°C

Dr. Miri Stolovich-Rain

Verified customer

Submitted Nov 09 2011

1-10 of 17 Abreviews or Q&A

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