Overview

  • Product name
    Anti-Glucagon antibody [K79bB10]
    See all Glucagon primary antibodies
  • Description
    Mouse monoclonal [K79bB10] to Glucagon
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, ICC/IF, IHC-Fr, RIA, Dot blotmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human, Pig, Common marmoset
    Predicted to work with: Rabbit, Guinea pig, Cat, Syrian hamster
  • Immunogen

    Polymerized porcine glucagon.

  • Positive control
    • Pancreatic glucagon for RIA.
  • General notes

    Production of this antibody has been changed on 8th July 2016. The following lots are from ascites and are still in stock as of 8th July 2016: GR260160 and GR268340.  Lot numbers higher than GR268340 will be purified from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab10988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 21821034
RIA Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.

Target

Images

  • ab10988 at 1/200 staining mouse pancreas tissue sections by IHC-P. The tissue sections were paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 16 hours at 4°C. A Texas Red conjugated donkey anti-mouse antibody was used as the secondary.

    See Abreview

  • Immunohistochemical analysis of Mouse pancreas tissue sections labeling Glucagon with ab10988 at 1/1000 dilution

    See Abreview

  • Immunohistochemical analysis of Mouse pancreas tissue labeling Glucagon with ab10988 at 1/1000 dilution.

    See Abreview

  • ab10988 staining Glucagon in rat pancreatic tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in paraformaldehyde, blocked with 2% serum for 1 hour at 25°C, then incubated with ab10988 at a 1/200 dilution for 1 hour at 25°C. The secondary used was a donkey anti-mouse IgG (H+L) conjugated to Texas Red, used at a 1/250 dilution. Nuclei counterstained with DAPI, green is staining for insulin.

    See Abreview

  • ab10988 staining human islets (beta cells) by ICC/IF.  Cells were PFA fixed and blocked in 2% serum for 1 hour at 25°C prior to incubating with ab10988 for 1 hour at 25°C.  A Cy3® conjugated goat anti-mouse antibody diluted 1/500 was used as the secondary.

    See Abreview

References

This product has been referenced in:
  • Al-Amily IM  et al. The functional impact of G protein-coupled receptor 142 (Gpr142) on pancreatic ß-cell in rodent. Pflugers Arch 471:633-645 (2019). Read more (PubMed: 30767071) »
  • Wang G  et al. Human amniotic epithelial cells regulate osteoblast differentiation through the secretion of TGFß1 and microRNA-34a-5p. Int J Mol Med 41:791-799 (2018). ICC/IF ; Human . Read more (PubMed: 29207015) »
See all 58 Publications for this product

Customer reviews and Q&As

11-19 of 19 Abreviews or Q&A

Question
Answer

Thank you for contacting us. I was able to find a paper describing production and characterization of the ab10988 clone, K79bB10, but as far as I can tell, the epitope has not been mapped. Here is a link to the paper's abstract: http://www.ncbi.nlm.nih.gov/pubmed/3138717 Production and use of monoclonal glucagon and insulin antibodies--reduction of pancreatic insulin in rats by treatment with complete Freund's adjuvant. Acta Histochem Suppl. 1988;35:217-23. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreas)
Specification
pancreas
Fixative
Formaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
(agent) for 10 minute(s) · Concentration: 100% · Temperature: RT°C

Dr. Miri Stolovich-Rain

Verified customer

Submitted Nov 09 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Pancreas)
Specification
Pancreas
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Mr. Thomas Schöppe

Verified customer

Submitted Jul 15 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Specification
Pancreas
Fixative
Formaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Feb 10 2009

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Islets (Beta cells))
Specification
Islets (Beta cells)
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Dr. Mallikarjuna Reddy Metukuri

Verified customer

Submitted Apr 21 2008

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreatic sections (Islet))
Specification
Pancreatic sections (Islet)
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Dr. Mallikarjuna Reddy Metukuri

Verified customer

Submitted Apr 18 2008

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreas)
Specification
pancreas
Fixative
Paraformaldehyde
Antigen retrieval step
None - Buffer/Enzyme Used: sodium citrate buffer
Permeabilization
Yes - 1% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Dr. Jiang Hu

Verified customer

Submitted Jun 05 2007

Answer

Thank you for your enquiry. The antibody ab10988 was raised against polymerized porcine glucagon, not a fragment of the polypeptide. The epitope it recognizes has not been mapped to a specific region; it may bind at the N-terminal but we cannot be sure.

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Answer

Thank you for your enquiry regarding ab12762(25µg), ab12764(25µg), ab12769(25µg), ab11022(1ml), ab10988(200µl) and ab7761(100µg). These products are all priced at $295 US dollars. In the future you can view pricing,availability and detailed information online by logging onto our website at www.Abcam.com. If you need additional information to that provided on the product datasheet, please contact us again and we will gladly assist you.

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11-19 of 19 Abreviews or Q&A

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