Question (44606) | Anti-Glucagon antibody [K79bB10] (ab10988)

Go to datasheet (ab10988)

Question

ab87108: ICC/IF image: why is the protein not localized in the nucleus, but rather cytosol or ER?
ab36232: ICC/IF protocol?
ab10988: blocking conditions, ICC/IF protocol?
ab7842: blocking conditions, ICC/IF protocol?

Answer

Thank you for contacting us.

For ab10988: ICC/IF was added based on published references and a customer Abreview. Thus, a protocol would be based on these sources. We have not tested it ourselves in this application.

The abreview information can be found here. This is all the information we have:
https://www.abcam.com/index.html?datasheet=10988&tab=abreviews&intabreviewid=10554

And the references are as follows:

1) Lu J et al. Alpha cell-specific Men1 ablation triggers the transdifferentiation of glucagon-expressing cells and insulinoma development. Gastroenterology 138:1954-65 (2010). ICC/IF, IHC-P; Mouse. PubMed: 20138042

2) Hanley S & Rosenberg L Transforming growth factor beta is a critical regulator of adult human islet plasticity. Mol Endocrinol 21:1467-77 (2007). ICC/IF; Human. PubMed: 17405902

I have not yet heard back yet from the lab regading the other antibodies, but will let you know as soon asI do.

Thank you for your patience.

I hope this information is of some help so far. In the meantime, please do not hesitate to contact us if you need any more advice or information.

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