Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucagon antibody [K79bB10]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Pancreas)
Specification
Pancreas
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Other product details

Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS, 2% Serum

Secondary antibody

Name
Non-Abcam antibody was used: TexasRed, Donkey @ mouse IgG (H+L) dianova
Host species: Donkey
Clonality: Polyclonal
Conjugation: Texas Red ®
Dilution
1/250

Additional data

Additional Notes
1µm sections,
blue-> nuclear dye DAPI
green->insulin
red-> glucagon

60x magni

Mr. Thomas Schöppe

Verified customer

Submitted Jul 15 2011

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