Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucagon antibody [K79bB10]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreas)
Specification
pancreas
Fixative
Formaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
(agent) for 10 minute(s) · Concentration: 100% · Temperature: RT°C

Other product details

Dilution
1/1000
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: Cy3 a mouse (?
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/500

Additional data

Additional Notes
Tissue fixation: 3-4h 4% buffered formaldehyde.
I also stained w/this ab 2h RT and than 2h secondary and the staining was nice.

Other channels:Dy488gpInsulin (Abcam) and TOTO3

Dr. Miri Stolovich-Rain

Verified customer

Submitted Nov 09 2011

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