Key features and details
- Rabbit polyclonal to Glucagon Receptor
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Glucagon Receptor antibody
See all Glucagon Receptor primary antibodies
DescriptionRabbit polyclonal to Glucagon Receptor
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Macaque monkey, Gorilla
Synthetic peptide corresponding to Human Glucagon Receptor aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following whole cell lysates: Caco2; HepG2; SW480.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab109279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).|
FunctionThis is a receptor for glucagon which plays a central role in regulating the level of blood glucose by controlling the rate of hepatic glucose production and insulin secretion. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.
Sequence similaritiesBelongs to the G-protein coupled receptor 2 family.
Cellular localizationCell membrane.
- Information by UniProt
- Gcgr antibody
- GGR antibody
- GL R antibody
All lanes : Anti-Glucagon Receptor antibody (ab109279) at 1/250 dilution (Milk Blocking - 3%)
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Additional bands at: 25 kDa, 51 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
The band observed at 51 kDa could potentially be a cleaved form of Glucagon Receptor due to the presence of a 25 amino acid signal peptide.
ab109279 has not yet been referenced specifically in any publications.