Product nameAnti-Glucagon Receptor antibody
See all Glucagon Receptor primary antibodies
DescriptionRabbit polyclonal to Glucagon Receptor
Tested applicationsSuitable for: WB, ELISA, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthesized peptide derived from internal of human Glucagon Receptor
- extracts from COLO205 cells; HeLa cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab75240 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 54 kDa.Can be blocked with Glucagon Receptor peptide (ab140072).|
|ICC/IF||1/500 - 1/1000.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionThis is a receptor for glucagon which plays a central role in regulating the level of blood glucose by controlling the rate of hepatic glucose production and insulin secretion. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.
Sequence similaritiesBelongs to the G-protein coupled receptor 2 family.
Cellular localizationCell membrane.
- Information by UniProt
- Gcgr antibody
- GGR antibody
- GL R antibody
All lanes : Anti-Glucagon Receptor antibody (ab75240) at 1/500 dilution
Lane 1 : extracts from COLO205 cells
Lane 2 : extracts from COLO205 cells with immunizing peptide at 5 µg
Lysates/proteins at 15 µg per lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Left panel: immunofluorescence analysis of HeLa cells with 1/500 ab75240. Right panel: same, after pre-incubation with the immunizing peptide.
IHC image of ab75240 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75240, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab75240 has been referenced in 1 publication.
- Quiñones M et al. Hypothalamic CaMKKß mediates glucagon anorectic effect and its diet-induced resistance. Mol Metab 4:961-70 (2015). WB . PubMed: 26909312