Key features and details
- Rabbit polyclonal to Glucocorticoid Receptor
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Glucocorticoid Receptor antibody
See all Glucocorticoid Receptor primary antibodies
DescriptionRabbit polyclonal to Glucocorticoid Receptor
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human Glucocorticoid Receptor. Synthesized non-phosphopeptide derived from Human Glucocorticoid Receptor around the phosphorylation site of Serine 211 (N-E-S-P-W).
Database link: P04150
- Recombinant Human Glucocorticoid Receptor protein (ab82089) can be used as a positive control in WB. K562 cell extract; Human breast carcinoma tissue; A549 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS (without Mg2+and Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab196944 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Our Abpromise guarantee covers the use of ab196944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 86 kDa.|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/500.|
FunctionReceptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation.
Tissue specificityWidely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.
Involvement in diseaseDefects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
modificationsIncreased proteasome-mediated degradation in response to glucocorticoids.
Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
Sumoylated; this reduces transcription transactivation.
Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling.
Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus.
- Information by UniProt
- GCCR antibody
- GCR antibody
- GCR_HUMAN antibody
All lanes : Anti-Glucocorticoid Receptor antibody (ab196944) at 1/500 dilution
Lane 1 : K562 cell extract with synthesized peptide
Lane 2 : K562 cell extract
Predicted band size: 86 kDa
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Glucocorticoid Receptor with ab196844 at 1/50 dilution. The image on the right has been pre-incubated with the blocking peptide.
Immunofluorescent analysis of A549 cells labeling Glucocorticoid Receptor with ab196944 at 1/100 dilution. The image on the right has been pre-incubated with the blocking peptide.
ab196944 has been referenced in 1 publication.
- Zheng LL et al. Preimplantation maternal stress impairs embryo development by inducing oviductal apoptosis with activation of the Fas system. Mol Hum Reprod 22:778-790 (2016). PubMed: 27475493