Overview

  • Product name

    Anti-Glucocorticoid Receptor antibody
    See all Glucocorticoid Receptor primary antibodies
  • Description

    Rabbit polyclonal to Glucocorticoid Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Glucocorticoid Receptor. Synthesized non-phosphopeptide derived from Human Glucocorticoid Receptor around the phosphorylation site of Serine 211 (N-E-S-P-W).
    Database link: P04150

  • Positive control

    • Recombinant Human Glucocorticoid Receptor protein (ab82089) can be used as a positive control in WB. K562 cell extract; Human breast carcinoma tissue; A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab196944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 86 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/500.

Target

  • Function

    Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation.
  • Tissue specificity

    Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.
  • Involvement in disease

    Defects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    modifications

    Increased proteasome-mediated degradation in response to glucocorticoids.
    Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
    Sumoylated; this reduces transcription transactivation.
    Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • GCCR antibody
    • GCR antibody
    • GCR_HUMAN antibody
    • GCRST antibody
    • glucocorticoid nuclear receptor variant 1 antibody
    • Glucocorticoid receptor antibody
    • GR antibody
    • GRL antibody
    • Grl1 antibody
    • nr3c1 antibody
    • Nuclear receptor subfamily 3 group C member 1 antibody
    • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody
    see all

Images

  • All lanes : Anti-Glucocorticoid Receptor antibody (ab196944) at 1/500 dilution

    Lane 1 : K562 cell extract with synthesized peptide
    Lane 2 : K562 cell extract

    Predicted band size: 86 kDa

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Glucocorticoid Receptor with ab196844 at 1/50 dilution. The image on the right has been pre-incubated with the blocking peptide.

  • Immunofluorescent analysis of A549 cells labeling Glucocorticoid Receptor with ab196944 at 1/100 dilution. The image on the right has been pre-incubated with the blocking peptide.

References

This product has been referenced in:

  • Zheng LL  et al. Preimplantation maternal stress impairs embryo development by inducing oviductal apoptosis with activation of the Fas system. Mol Hum Reprod 22:778-790 (2016). Read more (PubMed: 27475493) »
See 1 Publication for this product

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