Key features and details
- Rabbit polyclonal to Glucocorticoid Receptor
- Suitable for: WB, IP, IHC-P, ChIP-sequencing
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Glucocorticoid Receptor antibody
See all Glucocorticoid Receptor primary antibodies
DescriptionRabbit polyclonal to Glucocorticoid Receptor
Tested applicationsSuitable for: WB, IP, IHC-P, ChIP-sequencingmore details
Species reactivityReacts with: Human
Predicted to work with: Sheep
Synthetic peptide within Human Glucocorticoid Receptor aa 150-200. The exact sequence is proprietary. (NP_000167.1).
Database link: P04150
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate (ab150035). IHC-P: Human lung carcinoma tissue. ChIPseq: Prednisolone-treated THP-1 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab225886 was affinity purified using an epitope specific to Glucocorticoid Receptor immobilized on solid support.
Our Abpromise guarantee covers the use of ab225886 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 86 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ChIP-sequencing||Use 4 µg for 30 µg of chromatin.|
FunctionReceptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation.
Tissue specificityWidely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.
Involvement in diseaseDefects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
modificationsIncreased proteasome-mediated degradation in response to glucocorticoids.
Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
Sumoylated; this reduces transcription transactivation.
Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling.
Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus.
- Information by UniProt
- GCCR antibody
- GCR antibody
- GCR_HUMAN antibody
All lanes : Anti-Glucocorticoid Receptor antibody (ab225886) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 86 kDa
Exposure time: 30 seconds
Glucocorticoid Receptor was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225886 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225886 at 0.4 µg/ml.
Lane 1: ab225886 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 1 second.
Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for Glucocorticoid Receptor using ab225886 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
Chromatin from prednisolone treated THP-1 (human monocytic leukemia cell line) cells was immunoprecipitated with ab225886 and analyzed by DNA sequencing. The figure illustrates the peak distribution of Glucocorticoid Receptor binding within a 250 Kb region of chromosome 6 as detected using ab225886.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225886 has not yet been referenced specifically in any publications.