Overview

  • Product name

    Anti-Glucocorticoid Receptor antibody
    See all Glucocorticoid Receptor primary antibodies
  • Description

    Rabbit polyclonal to Glucocorticoid Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Sheep, Cow, Cat
  • Immunogen

    Recombinant fragment within Human Glucocorticoid Receptor (internal sequence). The exact sequence is proprietary.
    Database link: P04150

  • Positive control

    • WB: HeLa whole cell extract; Jurkat and Raji whole cell lysates. IP: HeLa whole cell extract. IHC-P: Human colon carcinoma tissue. ICC/IF: A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 86 kDa.
IP 1/500 - 1/1000.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation.
  • Tissue specificity

    Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.
  • Involvement in disease

    Defects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    modifications

    Increased proteasome-mediated degradation in response to glucocorticoids.
    Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
    Sumoylated; this reduces transcription transactivation.
    Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • GCCR antibody
    • GCR antibody
    • GCR_HUMAN antibody
    • GCRST antibody
    • glucocorticoid nuclear receptor variant 1 antibody
    • Glucocorticoid receptor antibody
    • GR antibody
    • GRL antibody
    • Grl1 antibody
    • nr3c1 antibody
    • Nuclear receptor subfamily 3 group C member 1 antibody
    • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody
    see all

Images

  • All lanes : Anti-Glucocorticoid Receptor antibody (ab227524) at 1/1000 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : Glucocorticoid Receptor Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 86 kDa

  • All lanes : Anti-Glucocorticoid Receptor antibody (ab227524) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : Glucocorticoid Receptor knockout HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 86 kDa



    7.5% SDS-PAGE gel.

  • All lanes : Anti-Glucocorticoid Receptor antibody (ab227524) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 86 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded human colon carcinoma tissue stained for Glucocorticoid Receptor using ab227524 at 1/250 dilution in immunohistochemical analysis.

  • Paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for Glucocorticoid Receptor (green) using ab227524 at 1/200 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

  • Glucocorticoid Receptor was immunoprecipitated from 1 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 2.5 µg of ab227524. Western blot was performed from the immunoprecipitate using ab227524 at 1/1000 dilution.

    Lane 1: HeLa whole cell lysate 40 µg (Input). 
    Lane 2: 2.5 µg preimmune rabbit IgG instead of ab227524 in HeLa whole cell lysate. 
    Lane 3: ab227524 IP in HeLa whole cell lysate. 

References

ab227524 has not yet been referenced specifically in any publications.

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