Anti-Glucocorticoid Receptor antibody (ab3578)
- Datasheet
- References (9)
- Protocols
Overview
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Product name
Anti-Glucocorticoid Receptor antibody
See all Glucocorticoid Receptor primary antibodies -
Description
Rabbit polyclonal to Glucocorticoid Receptor -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, Inhibition Assay, ChIP, GSA, IP, ICC, WB, IHC-FoFrmore details -
Species reactivity
Reacts with: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Human, Saccharomyces cerevisiae, Non human primates
Predicted to work with: Guinea pig, Pig
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Immunogen
Synthetic peptide corresponding to Human Glucocorticoid Receptor aa 346-367.
Sequence:DQKPIFNVIPPIPVGSENWNRC
(Peptide available asab5019)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 100% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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ChIP Related Products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab3578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/20. | |
| IHC-P | 1/200. | |
| Inhibition Assay | Use at an assay dependent concentration. | |
| ChIP | Use at an assay dependent concentration. | |
| GSA | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| ICC | Use a concentration of 1 µg/ml. Immunocytochemical staining of GR in HeLa cells results in cytoplasmic staining in the absence of ligand, and nuclear staining after hormone administration. |
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| WB | Use a concentration of 5 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 86 kDa).Can be blocked with Glucocorticoid Receptor peptide (ab5019). | |
| EMSA | Use at an assay dependent concentration. | |
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 18279320 |
Target
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Function
Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation. -
Tissue specificity
Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. -
Involvement in disease
Defects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Post-translational
modificationsIncreased proteasome-mediated degradation in response to glucocorticoids.
Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
Sumoylated; this reduces transcription transactivation.
Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2908 Human
- Entrez Gene: 14815 Mouse
- Entrez Gene: 24413 Rat
- Omim: 138040 Human
- SwissProt: P04150 Human
- SwissProt: P06537 Mouse
- SwissProt: P06536 Rat
- Unigene: 122926 Human
see all -
Alternative names
- GCCR antibody
- GCR antibody
- GCR_HUMAN antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3578)Immunocytochemistry/Immunofluorescence analysis of U251 cells labeling Glucocorticoid (green) with ab3578 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
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Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3578)Immunocytochemistry/Immunofluorescence analysis of NIH-3T3 cells labeling Glucocorticoid (green) with ab3578 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
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Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3578)Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling Glucocorticoid (green) with ab3578 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Glucocorticoid Receptor antibody (ab3578)This image is courtesy of an anonymous Abreviewab3578 staining Glucocorticoids Receptors in Mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue underwent fixation in 4% paraformaldehyde and 15µm sections were cut using cryostat. The primary antibody was diluted, 1/100 in TNB buffer and incubated with sample for 18 hours at 4°C. A HRP conjugated goat polyclonal to rabbit IgG, diluted 1/500 was used as secondary. Red staining repersents staining with ab3578 in perilesional cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody (ab3578)Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human cervical carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/200 with a rabbit polyclonal antibody recognizing Glucocorticoid Receptor (ab3578) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody (ab3578)Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/200 with a rabbit polyclonal antibody recognizing Glucocorticoid Receptor (ab3578) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Xia L et al. Rosiglitazone Improves Glucocorticoid Resistance in a Sudden Sensorineural Hearing Loss by Promoting MAP Kinase Phosphatase-1 Expression. Mediators Inflamm 2019:7915730 (2019). Read more (PubMed: 31217747) »
- Dayger CA et al. Physiological correlates of reproductive decisions: Relationships among body condition, reproductive status, and the hypothalamus-pituitary-adrenal axis in a reptile. Horm Behav 100:1-11 (2018). WB . Read more (PubMed: 29452075) »
