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    glucocorticoid-receptor-antibody-ab3578.pdf

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Signal Transduction Signaling Pathway Nuclear Signaling Nuclear Hormone Receptors Corticoid
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Anti-Glucocorticoid Receptor antibody (ab3578)

  • Datasheet
  • SDS
Reviews (4)Q&A (9)References (15)

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Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3578)
  • Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3578)
  • Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3578)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody (ab3578)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody (ab3578)

Key features and details

  • Rabbit polyclonal to Glucocorticoid Receptor
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Glucocorticoid Receptor antibody
    See all Glucocorticoid Receptor primary antibodies
  • Description

    Rabbit polyclonal to Glucocorticoid Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Guinea pig, Pig
  • Immunogen

    Synthetic peptide corresponding to Human Glucocorticoid Receptor aa 346-367.
    Sequence:

    DQKPIFNVIPPIPVGSENWNRC


    (Peptide available as ab5019)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • ICC: human HeLa, U251, mouse NIH-3T3 cells; IHC: human cervical carcinoma, tonsil tissues.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: 100% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Corticoid
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Corticoid
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Other

Associated products

  • Assay kits

    • Glucocorticoid Receptor Transcription Factor Assay Kit (Colorimetric) (ab207207)
  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Glucocorticoid Receptor protein (ab82089)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (1)
1/20.
IHC-P (1)
1/200.
Notes
ICC/IF
1/20.
IHC-P
1/200.

Target

  • Function

    Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation.
  • Tissue specificity

    Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.
  • Involvement in disease

    Defects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    modifications

    Increased proteasome-mediated degradation in response to glucocorticoids.
    Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
    Sumoylated; this reduces transcription transactivation.
    Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus.
  • Target information above from: UniProt accession P04150 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2908 Human
    • Entrez Gene: 14815 Mouse
    • Omim: 138040 Human
    • SwissProt: P04150 Human
    • SwissProt: P06537 Mouse
    • Unigene: 122926 Human
    • Unigene: 129481 Mouse
    • Alternative names

      • GCCR antibody
      • GCR antibody
      • GCR_HUMAN antibody
      • GCRST antibody
      • glucocorticoid nuclear receptor variant 1 antibody
      • Glucocorticoid receptor antibody
      • GR antibody
      • GRL antibody
      • Grl1 antibody
      • nr3c1 antibody
      • Nuclear receptor subfamily 3 group C member 1 antibody
      • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3578)
      Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3578)

      Immunocytochemistry/Immunofluorescence analysis of U251 cells labeling Glucocorticoid (green) with ab3578 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

    • Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3578)
      Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3578)

      Immunocytochemistry/Immunofluorescence analysis of NIH-3T3 cells labeling Glucocorticoid (green) with ab3578 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

    • Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3578)
      Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3578)

      Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling Glucocorticoid (green) with ab3578 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody (ab3578)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody (ab3578)

      Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human cervical carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/200 with a rabbit polyclonal antibody recognizing Glucocorticoid Receptor (ab3578) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody (ab3578)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucocorticoid Receptor antibody (ab3578)

      Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/200 with a rabbit polyclonal antibody recognizing Glucocorticoid Receptor (ab3578) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (15)

    Publishing research using ab3578? Please let us know so that we can cite the reference in this datasheet.

    ab3578 has been referenced in 15 publications.

    • Du S  et al. Does Maternal Stress Affect the Early Embryonic Microenvironment? Impact of Long-Term Cortisol Stimulation on the Oviduct Epithelium. Int J Mol Sci 21:N/A (2020). PubMed: 32284519
    • Chen J  et al. Paeoniflorin regulates the hypothalamic-pituitary-adrenal axis negative feedback in a rat model of post-traumatic stress disorder. Iran J Basic Med Sci 23:439-448 (2020). PubMed: 32489558
    • Wang N  et al. ACY-1215, a HDAC6 inhibitor, decreases the dexamethasone-induced suppression of osteogenesis in MC3T3-E1 cells. Mol Med Rep 22:2451-2459 (2020). PubMed: 32705192
    • Zhou H  et al. Endothelial cell-glucocorticoid receptor interactions and regulation of Wnt signaling. JCI Insight 5:N/A (2020). PubMed: 32051336
    • Seo JH  et al. Anti-inflammatory effect of Salsola komarovii extract with dissociated glucocorticoid activity. BMC Complement Med Ther 20:176 (2020). PubMed: 32503533
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 13 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucocorticoid Receptor antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (oviduct epithelia cultured on insert)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization
    No
    Specification
    oviduct epithelia cultured on insert
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Bouin´s solution and formaldehyde solution 4 %
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 17 2019

    Western blot abreview for Anti-Glucocorticoid Receptor antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Red sided garter snake Tissue lysate - whole (heart, brain, liver)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    5 µg
    Specification
    heart, brain, liver
    Blocking step
    5% milk + 1% BSA in Tris-buffered saline pH 7.4 as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 06 2016

    Question

    Inquiry: Hi, is the blocking peptide (imunogen) to ab3578 available? Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Feb 15 2013

    Answer

    Thank you for your enquiry and interest in our products.
    The immunogen sequence used to raise this antibody (ab3578: Anti-Glucocorticoid Receptor) was DQKPIFNVIPPIPVGSENWNR, corresponding to amino acids 346-367 of Human Glucocorticoid Receptor. However, currently this peptide is not in our catalogue.
    Apologies!
    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Feb 15 2013

    Question

    Thanks a lot for the quick response.
    I would keep you informed about the observations.
    Adding up to the previous results I would also like to inform you that I gave another try in IHC including this time a citrate buffer antigen retreival step and still the result was not the usual expected one. I attach here the image obtained. In green are the pyramidal neurons of CA1- CA2 region of hippocampus and red depicts the GR antibody wich should show strong nuclear localization in CA1 layer.
    Hope that would be useful to you :-)

    Read More

    Abcam community

    Verified customer

    Asked on May 24 2012

    Answer

    Thank you very much for this extra information, this is very useful indeed for our investigations.

    Please do not hesitate to inform me if the replacement antibody gave you satisfactory results or not.

    Read More

    Abcam Scientific Support

    Answered on May 24 2012

    Question

    I would appreciate a replacement with ab 3579

    Read More

    Abcam community

    Verified customer

    Asked on May 23 2012

    Answer

    Thank you foryour cooperation.

    I am sorry ab3578 did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with one unit of ab3579. The order number ****.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on May 23 2012

    Question

    Description of the problem : multiple bands and no strong band in the
    expected molecular weight
    Sample (mouse brain supernatant)
    Sample preparation (SDS buffer, Protease inhibitors, Heating sample)
    Amount of protein loaded: 20ug
    Electrophoresis/Gel conditions (10% SDS gel)
    Transfer and blocking conditions (transfer Buffer with methanol/trans
    blot turbo bio rad, blocked in 5% BSA for 1 hour)
    Primary Antibody (abcam/rabbit/Diluent BSA/Dilution 1:2000/overnight
    incubation, TBST 4 washes 10 min each)
    Secondary Antibody (jacksons/goat anti rabbit/Diluent BSA/Dilution
    1:5000/1 hour incubation, wash with TBST 4 times)
    Detection method (ECL millipore)
    How many times have you tried the Western? 3 times
    Have you run a "No Primary" control?
    No
    Do you obtain the same results every time?
    Yes
    e.g. are the background bands always in the same place? yes
    I have also used it in IHC with PFA fixed sagittal mouse brain sections
    at 1:200 dilution with no results.

    Read More

    Abcam community

    Verified customer

    Asked on May 21 2012

    Answer

    Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

    Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

    I apologise for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation.

    Please note that the only lot we currently have in stock for ab3578 is the same as the one you received and tested (lot # GR76455). Therefore I can offer you as a replacement an alternative anti-Glucocorticoid Receptor from the following list :



    https://www.abcam.com/Glucocorticoid-Receptor-antibody-ChIP-Grade-ab3579.html , Rabbit polyclonal




    https://www.abcam.com/Glucocorticoid-Receptor-phospho-S226-antibody-ab53692.html https://www.abcam.com/Glucocorticoid-Receptor-phospho-S226-antibody-ab53692.html Rabbit polyclonal




    https://www.abcam.com/Glucocorticoid-Receptor-antibody-ab16510.html, Rabbit polyclonal


    , Rabbit polyclonal

    , Rabbit monoclonal
    https://www.abcam.com/Glucocorticoid-Receptor-antibody-BuGR2-ab2768.html, Mouse monoclonal



    https://www.abcam.com/Glucocorticoid-Receptor-antibody-ab55400.html
    https://www.abcam.com/Glucocorticoid-Receptor-antibody-EPR4595-ab109022.html



    Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Abcam Scientific Support

    Answered on May 21 2012

    Question

    Using ab3578 in WB on mouse brain
    Obtaining a band at the incorrect size of 50 kDa.

    Read More

    Abcam community

    Verified customer

    Asked on May 04 2012

    Answer

    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for WBand Mouse, Rat, Hamster and Human samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

    I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

    I would appreciate if you are also able to provide an image which would help us to assess the results.

    Thank you for your time and cooperation. We look forward to receiving the completed questionaire.



    Order Details
    Antibody code:

    Problem
    Choose: Non-specific band Multiple bands No signal or weak signal High background

    Lot number

    Purchase order number
    or preferably Abcam order number:



    General Information
    Antibody storage conditions (temperature/reconstitution etc)


    Description of the problem (high background, wrong band size, more bands, no band etc.)


    Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)


    Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)


    Amount of protein loaded


    Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)


    Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)


    Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


    Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


    Detection method (ECL, ECLPlus etc.)


    Positive and negative controls used (please specify)



    Optimization attempts (problem solving)
    How many times have you tried the Western?



    Have you run a "No Primary" control?
    Yes No

    Do you obtain the same results every time?
    Yes No
    e.g. are the background bands always in the same place?


    What steps have you altered?


    Additional Notes:


    Image:
    We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to asess the results.

    Read More

    Abcam Scientific Support

    Answered on May 04 2012

    Question

        Hi John, Thanks for your email and reply; I apologize for the extra email.   That’s weird, though, because I didn’t see that email come through for me on Monday...who knows what happened.   You’re correct, it was my typo and the antibody catalogue number should be AB3578 (x2).

    Read More

    Abcam community

    Verified customer

    Asked on Dec 15 2011

    Answer

    I just went back and checked, when I thought I was replying to you, I was actually replying to the customer. Sorry about that. I have processed the two credits for ab3578. If there is anything else I can help you with, please let me kn

    Read More

    Abcam Scientific Support

    Answered on Dec 15 2011

    Question

    Hello Abcam,   Hope everyone’s had a nice week.   I just realized I sent this to the wrong email address.  Could someone look into the below?  

    Read More

    Abcam community

    Verified customer

    Asked on Dec 14 2011

    Answer

    I got the email you sent on Monday, the customer must have forwarded it to me. Please see below for what I replied with on Monday: "Thank you for your reply. Before I issue the credit, I just want to confirm that we are talking about the correct antibody. I thought we were talking about ab3578, however in the last email you mentioned ab3574. I am sure it was just a typo, but I wanted to double check. Also could you provide me with the customers Cedarlane number, so that I can add that information to the credit note for ease of tracking. I look forward to your reply." If you could just clarify the issue above, I can go ahead and process the credit.  

    Read More

    Abcam Scientific Support

    Answered on Dec 14 2011

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Glucocorticoid Receptor antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (brain)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    brain
    Blocking step
    Perkin-Elmer TNB Blocking Reagent as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 23 2009

    1-10 of 13 Abreviews or Q&A

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