Product nameAnti-Glucocorticoid Receptor antibody - ChIP Grade
See all Glucocorticoid Receptor primary antibodies
DescriptionRabbit polyclonal to Glucocorticoid Receptor - ChIP Grade
Tested applicationsSuitable for: ChIP, Flow Cyt, ICC, IP, WB, IHC-Fr, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human, Non human primates
Predicted to work with: Rabbit, Guinea pig
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: 100% PBS
Concentration information loading...
PurityImmunogen affinity purified
ChIP Related Products
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab3579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|EMSA||Use at an assay dependent concentration.|
|ChIP||Use at an assay dependent concentration. See Lerner et al., 2003.|
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC||Use at an assay dependent concentration.|
|IP||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 5 µg/ml. Predicted molecular weight: 97 kDa.Can be blocked with Glucocorticoid Receptor peptide (ab5833). Detects an ~97 kDa protein representing GR as well as two other unidentified proteins at ~135 to ~145 kDa from rat liver extract. These cross reacting proteins are not believed to be GR or GR precursors as they do not bind [3H]dexamethasone mesylate.|
|IHC-Fr||Use a concentration of 1 µg/ml. Staining of GR in rat hippocampus results in staining primarily in the nucleus.|
|ICC/IF||1/40. PubMed: 19202080|
FunctionReceptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation.
Tissue specificityWidely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.
Involvement in diseaseDefects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
modificationsIncreased proteasome-mediated degradation in response to glucocorticoids.
Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
Sumoylated; this reduces transcription transactivation.
Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling.
Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus.
- Information by UniProt
- GCCR antibody
- GCR antibody
- GCR_HUMAN antibody
All lanes : Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3579) at 1 µg/ml
Lane 1 : T47D cell lysate with 5% Milk in TBST
Lane 2 : A594 cell lysate with 5% Milk in TBST
Lane 3 : K562 cell lysate with 5% Milk in TBST
Lane 4 : MCF7 cell lysate with 5% Milk in TBST
Lane 5 : Jurkat cell lysate with 5% Milk in TBST
All lanes : HRP conjugate at 1/1000 dilution
Predicted band size: 97 kDa
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling Glucocorticoid Receptor (green) with ab3579 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
Immunocytochemistry/Immunofluorescence analysis of A2058 cells labeling Glucocorticoid Receptor (green) with ab3579 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
This product has been referenced in:
- Tobin SW et al. Regulation of Hspb7 by MEF2 and AP-1: implications for Hspb7 in muscle atrophy. J Cell Sci 129:4076-4090 (2016). Read more (PubMed: 27632998) »
- Li Y et al. IL-7 Receptor Mutations and Steroid Resistance in Pediatric T cell Acute Lymphoblastic Leukemia: A Genome Sequencing Study. PLoS Med 13:e1002200 (2016). WB ; Human . Read more (PubMed: 27997540) »