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Thanks a lot for the quick response.
I would keep you informed about the observations.
Adding up to the previous results I would also like to inform you that I gave another try in IHC including this time a citrate buffer antigen retreival step and still the result was not the usual expected one. I attach here the image obtained. In green are the pyramidal neurons of CA1- CA2 region of hippocampus and red depicts the GR antibody wich should show strong nuclear localization in CA1 layer.
Hope that would be useful to you :-)
Asked on May 24 2012