Anti-Glucocorticoid Receptor (phospho S211) antibody (ab55189)
Key features and details
- Rabbit polyclonal to Glucocorticoid Receptor (phospho S211)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Glucocorticoid Receptor (phospho S211) antibody
See all Glucocorticoid Receptor primary antibodies -
Description
Rabbit polyclonal to Glucocorticoid Receptor (phospho S211) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human Glucocorticoid Receptor (phospho S211).
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Positive control
- Human breast carcinoma tissue, extracts from HeLa cells treated with PMA
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab55189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/500 - 1/1000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
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Notes |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/500 - 1/1000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa). |
Target
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Function
Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation. -
Tissue specificity
Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. -
Involvement in disease
Defects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Post-translational
modificationsIncreased proteasome-mediated degradation in response to glucocorticoids.
Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
Sumoylated; this reduces transcription transactivation.
Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2908 Human
- Entrez Gene: 14815 Mouse
- Entrez Gene: 24413 Rat
- Omim: 138040 Human
- SwissProt: P04150 Human
- SwissProt: P06537 Mouse
- SwissProt: P06536 Rat
- Unigene: 122926 Human
see all -
Alternative names
- GCCR antibody
- GCR antibody
- GCR_HUMAN antibody
see all
Images
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All lanes : Anti-Glucocorticoid Receptor (phospho S211) antibody (ab55189) at 1/500 dilution
Lane 1 : extracts from HeLa cells treated
with PMA (125ng/ml, 30min), with the immunising phosphpeptide.
Lane 2 : extracts from HeLa cells treated
with PMA (125ng/ml, 30min).
Predicted band size: 86 kDa
Observed band size: 86 kDa -
Ab55189 at 1/50 dilution staining human breast carcinoma without (left) and with (right) immunizing phospho-peptide; paraffin embedded.
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab55189 has been referenced in 8 publications.
- Choi D et al. ß-Ionone Attenuates Dexamethasone-Induced Suppression of Collagen and Hyaluronic Acid Synthesis in Human Dermal Fibroblasts. Biomolecules 11:N/A (2021). PubMed: 33919331
- Gorbacheva AM et al. Glucocorticoid Receptor Binding Inhibits an Intronic IL33 Enhancer and is Disrupted by rs4742170 (T) Allele Associated with Specific Wheezing Phenotype in Early Childhood. Int J Mol Sci 19:N/A (2018). PubMed: 30544846
- Rider CF et al. Long-Acting ß2-Adrenoceptor Agonists Enhance Glucocorticoid Receptor (GR)-Mediated Transcription by Gene-Specific Mechanisms Rather Than Generic Effects via GR. Mol Pharmacol 94:1031-1046 (2018). PubMed: 29959223
- Ryu JS et al. Prednisolone induces apoptosis in corneal epithelial cells through the intrinsic pathway. Sci Rep 7:4135 (2017). WB ; Human . PubMed: 28646191
- Pazdrak K et al. Cytokine-Induced Glucocorticoid Resistance from Eosinophil Activation: Protein Phosphatase 5 Modulation of Glucocorticoid Receptor Phosphorylation and Signaling. J Immunol 197:3782-3791 (2016). PubMed: 27742828
- Li X et al. Glucocorticoid receptor is involved in the breed-dependent transcriptional regulation of 3ß-hydroxysteroid dehydrogenase in the liver of preweaning piglets. BMC Vet Res 11:123 (2015). WB . PubMed: 26008782
- Jacob W et al. Decreased adipogenesis and adipose tissue in mice with inactivated protein phosphatase 5. Biochem J 466:163-76 (2015). PubMed: 25437352
- Goleva E et al. IFN{gamma} Reverses IL-2 and IL-4 Mediated T cell Steroid Resistance. Am J Respir Cell Mol Biol : (2008). PubMed: 18776133