Overview

  • Product name
    Anti-Glucocorticoid Receptor (phospho S211) antibody
    See all Glucocorticoid Receptor primary antibodies
  • Description
    Rabbit polyclonal to Glucocorticoid Receptor (phospho S211)
  • Host species
    Rabbit
  • Specificity
    Detects endogenous levels of the Glucocorticoid Receptor only when phosphorylated at serine 211 (Mouse Ser220, Rat Ser232)
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from the human Glucocorticoid Receptor around the phosphorylation site of serine 211 (NESPPW).

  • Positive control
    • Human breast carcinoma tissue, extracts from HeLa cells treated with PMA

Properties

Applications

Our Abpromise guarantee covers the use of ab55189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
ELISA 1/10000.

Target

  • Function
    Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation.
  • Tissue specificity
    Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.
  • Involvement in disease
    Defects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    modifications
    Increased proteasome-mediated degradation in response to glucocorticoids.
    Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
    Sumoylated; this reduces transcription transactivation.
    Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling.
  • Cellular localization
    Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • GCCR antibody
    • GCR antibody
    • GCR_HUMAN antibody
    • GCRST antibody
    • glucocorticoid nuclear receptor variant 1 antibody
    • Glucocorticoid receptor antibody
    • GR antibody
    • GRL antibody
    • Grl1 antibody
    • nr3c1 antibody
    • Nuclear receptor subfamily 3 group C member 1 antibody
    • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody
    see all

Images

  • All lanes : Anti-Glucocorticoid Receptor (phospho S211) antibody (ab55189) at 1/500 dilution

    Lane 1 : extracts from HeLa cells treated
    with PMA (125ng/ml, 30min), with the immunising phosphpeptide.
    Lane 2 : extracts from HeLa cells treated
    with PMA (125ng/ml, 30min).

    Predicted band size: 86 kDa
    Observed band size: 86 kDa

  • Ab55189 at 1/50 dilution staining human breast carcinoma without (left) and with (right) immunizing phospho-peptide; paraffin embedded.

References

This product has been referenced in:
  • Ryu JS  et al. Prednisolone induces apoptosis in corneal epithelial cells through the intrinsic pathway. Sci Rep 7:4135 (2017). WB ; Human . Read more (PubMed: 28646191) »
  • Pazdrak K  et al. Cytokine-Induced Glucocorticoid Resistance from Eosinophil Activation: Protein Phosphatase 5 Modulation of Glucocorticoid Receptor Phosphorylation and Signaling. J Immunol 197:3782-3791 (2016). Read more (PubMed: 27742828) »
See all 5 Publications for this product

Customer reviews and Q&As

Answer

I have been informed that rat tissue was not tested by the laboratory, but that it is predicted to cross react due to the high homology of the immunogen and the rat protein. I have therefore removed this species from the datasheet and arranged a credit note for this product (CN 2492), can you please offer a refund to the customer and my apologies for this error on the datasheet. I was also able to find out the specific IHC protocol used to test this antibody, I have enclosed it below for your customer if he/she would like to try it. Deparaffinization 1. Incubate slide at 60C for 60 minutes. 2. Deparaffinize in Xylene for 10 minutes and repeat one more times. 3. Hydrate in 100% alcohol for 5 minutes, in 95% alcohol for 5 minutes, in 85% alcohol for 5 minutes, in 75% alcohol for 5 minutes. 4. Dip into Distill Water for 5 minutes. 5. Dip into TBS (50 mM Tris, 100 mM NaCl, pH 7.6), leave for 5 minutes, and repeat two times. Antigen Retrieval 6. Bring 500 - 2000 ml 10 mM citrate buffer (pH6.0) to the boil in a stainless steel pressure cooker. 7. Put the slide into staining rack and lower into pressure cooker ensuring the slide is well immersed in citrate buffer. 8. When the pressure indicator valve has risen after 3-4 minutes, incubate for 1 minute. 9. Cool the slide naturally to room temperature. 10. Dip into distilled water, leave for 5 minutes, and repeat two times. 11. Dip the slide in TBS for 5 minutes and repeat two times. 12. Immerse slides in 3% H2O2 ( in fresh methanol) for 15 minutes at room temperature. 13. Wash with distilled water two times, 5 minutes each time. 14. Wash with TBS (pH 7.6) two times, 5 minutes each time. Staining with Primary Antibody 15. Dilute primary antibody with 3% BSA in TBS. Cover the tissue section on the slide with diluted primary antibody (use 50 – 150µl for each slide). 16. Incubate at 37C for 30 minutes or at room temperature for 60 minutes (The optimal incubation time, incubation temperature, and antibody dilution should be determined by the individual laboratory). 17. Wash with TBS two times, 5 minutes each time. Staining with Secondary Antibody 18. Incubate with 100-200µl Polymer Enhancer. Incubate 30 minutes at 37C. 19. Wash with TBS for 3 times, 5 minutes each time. 20. Incubate with 100-200µl Polymerized HRP and incubate 30 minutes at 37C. 21. Wash with TBS for 3 times, 5 minutes each time. 22. Add DAB solution and incubate 3-10 minutes(The reaction progress and the optimal time should be determine according to microscope). 23. Wash with distilled water for 2 times, 5 minutes each time. 24. Counterstain sections in hematoxylin if required,wash with distilled water.Immerse slides in 0.1% HCl- ethanol for 1-10 seconds, wash with distilled water. 25. Dehydrate through 95% ethanol for 1 minute, 100% ethanol for 2×3min, Xylene for 2×3min, and coverslip with mounting medium.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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