Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22216-414] to Glucocorticoid Receptor (phospho S226)
- Suitable for: WB, IHC-P, Dot blot
- Reacts with: Human
Product nameAnti-Glucocorticoid Receptor (phospho S226) antibody [EPR22216-414]
See all Glucocorticoid Receptor primary antibodies
DescriptionRabbit monoclonal [EPR22216-414] to Glucocorticoid Receptor (phospho S226)
Tested applicationsSuitable for: WB, IHC-P, Dot blotmore details
Unsuitable for: ChIP,Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Human
Synthetic peptide within Human Glucocorticoid Receptor aa 200-300. The exact sequence is proprietary.
Database link: P04150
- WB: U-2 OS treated with 1 µM Dexamethasone for 1hour whole cell lysate 20 µg (Untreated membrane), cell lysate. Dot blot: Glucocorticoid Receptor (phospho S226) peptide (aa220-231) and Glucocorticoid Receptor (phospho S226) peptide (aa225-234). IHC-P: Human endometrial cancer tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab228972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 85 kDa.
FunctionReceptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation.
Tissue specificityWidely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.
Involvement in diseaseDefects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
modificationsIncreased proteasome-mediated degradation in response to glucocorticoids.
Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
Sumoylated; this reduces transcription transactivation.
Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling.
Cellular localizationCytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus.
- Information by UniProt
- GCCR antibody
- GCR antibody
- GCR_HUMAN antibody
All lanes : Anti-Glucocorticoid Receptor (phospho S226) antibody [EPR22216-414] (ab228972) at 1/1000 dilution
Lane 1 : Untreated U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate (Untreated membrane)
Lane 2 : U-2 OS treated with 1 µM Dexamethasone for 1hour whole cell lysate 20 µg (Untreated membrane)
Lane 3 : U-2 OS treated with 1 µM Dexamethasone for 1hour whole cell lysate 20 µg (Phosphatase treated membrane
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 85 kDa
Observed band size: 86 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Glucocorticoid receptor is phosphorylated upon stimulation of dexamethasone, a corticosteroid (PMID: 28781762). Exposure time: 3 minutes
Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling Glucocorticoid Receptor (phospho S226) with ab228972 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human endometrium cancer (panel A), and no staining after alkaline phosphatase treatment (panel B) (PMID:12351702, 12225995). The section was incubated with ab228972 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Dot blot analysis using ab228972 at 1/1000 dilution. The secondary antibody was a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.
Lane 1: Glucocorticoid Receptor (phospho S226) peptide (aa220-231)
Lane 2: Glucocorticoid Receptor (phospho S226) peptide (aa225-234)
Lane 3: Glucocorticoid Receptor non-phospho peptide (aa220-234)
Blocking/diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3 minutes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228972 has not yet been referenced specifically in any publications.