Overview

  • Product name

  • Description

    Rabbit polyclonal to Glucokinase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human Glucokinase aa 1-250.
    Database link: P35557

  • Positive control

    • WB: HEK-293T, Huh7, Hep3B, A431, H1299, HeLa, HepG2, Molt-4, and Raji whole cell lysate. IHC-P: MCF7 xenograft. ICC/IF: A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab137714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage.
  • Tissue specificity

    Isoform 1 is expressed in pancreas. Isoform 2 and isoform 3 is expressed in liver.
  • Involvement in disease

    Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) [MIM:125851]; also shortened MODY-2. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
    Defects in GCK are the cause of familial hyperinsulinemic hypoglycemia type 3 (HHF3) [MIM:602485]; also known as persistent hyperinsulinemic hypoglycemia of infancy (PHHI) or congenital hyperinsulinism. HHF is the most common cause of persistent hypoglycemia in infancy. Unless early and aggressive intervention is undertaken, brain damage from recurrent episodes of hypoglycemia may occur.
  • Sequence similarities

    Belongs to the hexokinase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP:D-hexose 6-phosphotransferase antibody
    • FGQTL3 antibody
    • GCK antibody
    • GK antibody
    • GLK antibody
    • Glucokinase antibody
    • Hexokinase D pancreatic isozyme antibody
    • Hexokinase type IV antibody
    • Hexokinase-4 antibody
    • Hexokinase-D antibody
    • HHF3 antibody
    • HK IV antibody
    • HK4 antibody
    • HKIV antibody
    • HXK4_HUMAN antibody
    • HXKP antibody
    • LGLK antibody
    • MODY2 antibody
    see all

Images

  • All lanes : Anti-Glucokinase antibody (ab137714) at 1/1000 dilution

    Lane 1 : Huh7 whole cell lysate
    Lane 2 : Hep3B whole cell lysate
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 52 kDa

  • Paraffin-embedded human MCF7 xenograft tissue stained for Glucokinase using ab137714 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-Glucokinase antibody (ab137714) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 52 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed A549 cells labelling Glucokinase with ab137714 at 1/200 (panel 1) and co-stained with a DNA probe (panel 2).

References

This product has been referenced in:

  • Bassoy EY  et al. ER-mitochondria contacts control surface glycan expression and sensitivity to killer lymphocytes in glioma stem-like cells. EMBO J 36:1493-1512 (2017). Read more (PubMed: 28283580) »
See 1 Publication for this product

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