• Product name

    Anti-Glucokinase antibody - N-terminal
    See all Glucokinase primary antibodies
  • Description

    Rabbit polyclonal to Glucokinase - N-terminal
  • Host species

  • Tested applications

    Suitable for: ELISA, ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Pig, Common marmoset
  • Immunogen

    Synthetic peptide corresponding to Human Glucokinase (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P35557
    (Peptide available as ab108095)

  • Positive control

    • Human breast carcinoma and hepatocarcinoma tissues; HepG2 and mouse liver tissue cell lysates.



Our Abpromise guarantee covers the use of ab37796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB 1/100 - 1/500. Detects a band of approximately 65 kDa (predicted molecular weight: 52 kDa).
IHC-P 1/50 - 1/100.


  • Function

    Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage.
  • Tissue specificity

    Isoform 1 is expressed in pancreas. Isoform 2 and isoform 3 is expressed in liver.
  • Involvement in disease

    Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) [MIM:125851]; also shortened MODY-2. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
    Defects in GCK are the cause of familial hyperinsulinemic hypoglycemia type 3 (HHF3) [MIM:602485]; also known as persistent hyperinsulinemic hypoglycemia of infancy (PHHI) or congenital hyperinsulinism. HHF is the most common cause of persistent hypoglycemia in infancy. Unless early and aggressive intervention is undertaken, brain damage from recurrent episodes of hypoglycemia may occur.
  • Sequence similarities

    Belongs to the hexokinase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP:D-hexose 6-phosphotransferase antibody
    • FGQTL3 antibody
    • GCK antibody
    • GK antibody
    • GLK antibody
    • Glucokinase antibody
    • Hexokinase D pancreatic isozyme antibody
    • Hexokinase type IV antibody
    • Hexokinase-4 antibody
    • Hexokinase-D antibody
    • HHF3 antibody
    • HK IV antibody
    • HK4 antibody
    • HKIV antibody
    • HXK4_HUMAN antibody
    • HXKP antibody
    • LGLK antibody
    • MODY2 antibody
    see all


  • All lanes : Anti-Glucokinase antibody - N-terminal (ab37796)

    Lane 1 : Hep G2 cell lysate
    Lane 2 : Mouse liver lysate

    Predicted band size: 52 kDa
    Observed band size: 65 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 30 kDa, 37 kDa, 50 kDa. We are unsure as to the identity of these extra bands.

  • Formalin fixed, paraffin embedded human breast carcinoma tissue using ab37796, followed by AEC staining.
  • ICC/IF image of ab37796 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37796, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Taneera J  et al. GNAS gene is an important regulator of insulin secretory capacity in pancreatic ß-cells. Gene 715:144028 (2019). Read more (PubMed: 31374326) »
  • Shin H  et al. Blockade of cannabinoid 1 receptor improves glucose responsiveness in pancreatic beta cells. J Cell Mol Med 22:2337-2345 (2018). WB ; Mouse . Read more (PubMed: 29431265) »
See all 16 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A


The current lot we have in stock for ab37796 is GR8879-26. This has a concentration of 2 mg/ml.

Once you receive your order, if you have a different lot please do not hesitate to let me know and we can check the concentration for you.

Read More
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Human Cell lysate - whole cell (HepG2)
+/- Testosterone
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Sep 06 2013


Thank you for contacting us and for reporting the problem you experienced with the antibody ab37796. We will use the data you provided to investigate the source of the problem with this product. I am sorry that the product you received did not perform as stated on the datasheet. As requested, I am happy to issue a refund against the original order. In order to create this credit note / refund, could you please send me one of the following : the order number, the PO number and the date of purchase? Thank you.

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Merci de votre intérêt pour les anticorps Abcam. Oui, je recommanderais ab37796 pour le marquage de la glucokinase et ab69508 pour le récepteur insuline beta. Par contre, pour le marquage de PCK1 je recommanderais plutôt un anticorps testé en IHC-P tel que ab28455 (www.abcam.com/ab28455) ou ab115693 (www.abcam.com/ab115693). Concernant les deux anti-PCK1, à ma connaissance ces anticorps n’ont pas encore été testés chez la souris et nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires. Cependant, si vous souhaitez tester ab28455 ou ab115693 chez la souris, il me sera possible de vous offrir une remise. Après soumission d’une Abreview avec vos résultats, une remise correspondant à la valeur d’achat de ab28455 ou ab115693 pourra être utilisable lors d’une prochaine commande d’anticorps primaire. Etapes à suivre : 1. Nous confirmer que vous souhaiteriez tester ab28455 ou ab115693 chez la souris afin de recevoir le code de remise correspondant. Ce code doit être édité avant l’achat de ab28455 ou ab115693. 2. Acheter ab28455 ou ab115693 par téléphone, fax ou internet (www.abcam.com) 3. Le tester chez la souris 4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview. Pour plus d’informations https://www.abcam.com/abreviews 5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable lors de l’achat d’un autre anticorps primaire. Même si ab28455 ou ab115693 ne pas fonctionne pas chez la souris, après soumission de votre Abreview, vous recevrez quand même une remise sur l’achat de votre prochain anticorps. N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle. Termes et Conditions : www.abcam.com/collaborationdiscount.

Read More
Western blot
Rat Cell lysate - whole cell (pancreatic beta cells)
Loading amount
20 µg
pancreatic beta cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Dr. Sarah Ronnebaum

Verified customer

Submitted Jun 19 2007


Thank you for your patience in this matter. I have received a reply from the originator of ab37796 (IGLK antibody). Glucokinase is actually expressed in HepG2, please follow the links below to a couple of articles that demonstrate this point: http://www.biochemj.org/bj/333/0705/bj3330705.htm. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11460882&dopt=Abstract ab37796 was tested in WB by running sera from pre-immunized animals (Lane 1), sera from immunized animals(Lane 2), and purified antibody (Lane 3). A band was detected in lanes 2 and 3 but absent from lane 1. I hope this information helps. Please don't hesitate to contact us again if you have further questions.

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