Overview

  • Product name

    Gluconokinase Activity Assay Kit (Colorimetric)
  • Detection method

    Colorimetric
  • Sample type

    Cell Lysate, Tissue Lysate
  • Assay type

    Enzyme activity (quantitative)
  • Sensitivity

    < 100 µU
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Gluconokinase Activity Assay Kit (Colorimetric) (ab211092) provides a simple, sensitive and convenient method for detecting gluconokinase activity in a variety of samples such as cell and tissue lysates, and even in prokaryotes such as E. coli. In this assay, Gluconokinase converts Gluconate into 6-Phosphate-D-Gluconate in an ATP dependent manner. 6-Phosphate-D-Gluconate and ADP in turn undergo a series of reactions to form an intermediate, which reacts with the probe to form a colored product with strong absorbance (OD 450 nm). The detection limit of this assay < 0.1 mU.


     

  • Notes

    Gluconokinase (GntK, ATP:D-gluconate 6-phosphotransferase, Gluconate Kinase; EC:2.7.1.12) is a key enzyme for degradation of gluconate. In E. coli and yeast, Gluconokinase phosphorylates gluconate into 6-Phosphate-D-Gluconate in an ATP dependent manner. Via the Hexose Monophosphate Shunt (HMS) pathway, 6- Phosphate-D-Gluconate generates ribose-5-phosphate, which is critical for nucleotides and nucleic acid synthesis.

    Despite the widespread use of D-gluconate as acidity regulator in food and medicine, little is known of the mechanism of gluconate metabolism in humans.

  • Platform

    Microplate reader

Properties

Images

  • Typical NADH standard calibration curve.

  • Kinetic curves showing Gluconokinase activity detection in positive control (1 µL; included in kit), lysates from rat liver and kidkey (1 µg, respectively), lysates from MDA-MB-468 cells (1 µg) and E. coli (0.1 µg).

  • Gluconokinase specific activity in lysates from rat liver and kidkey (1 µg, respectively), lysates from MDA-MB-468 cells (1 µg) and E. coli (0.1 µg).

Protocols

References

ab211092 has not yet been referenced specifically in any publications.

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