Overview

  • Product name

    Glucose-1-Phosphate Assay Kit (Colorimetric)
  • Detection method

    Colorimetric
  • Sample type

    Tissue, Adherent cells, Suspension cells, Tissue Extracts
  • Assay type

    Quantitative
  • Sensitivity

    = 1 µM
  • Range

    1 µM - 10000 µM
  • Assay time

    0h 40m
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    In Glucose-1-phosphate Assay Kit (ab155892), G1P (glucose-1-phosphate) is converted to glucose-6-phosphate by phosphoglucomutase in the presence of Glucose 1,6-biphosphate; glucose-6-phosphate is subsequently oxidized by glucose-6-phosphate dehydrogenase to form NADH which reduces a colorless probe to a colored product with strong absorbance at 450 nm. Abcam's Glucose-1-phosphate Assay Kit is rapid, sensitive and easy to use and can detect 1µM - 10 mM G1P. This assay kit can be used for a variety of sample types.

  • Notes

    Glucose-1-phosphate (G1P) is an important carbohydrate intermediate in glucose metabolism and storage. In response to hormonal or neural signals, glycogenolysis occurs in liver and muscle tissues where Glucose-1-phosphate is released as the rate-limiting step in glycogen breakdown. Glucose-1-phosphate is subsequently converted to Glucose-6-phosphate by phosphoglucomutase and enters glycolysis. In glycogen synthesis, glucose is transferred to glycogen through the actions of Phosphoglucose isomerase (G6P→ G1P), UDPG-pyrophosphorylase (G1P → UDP-glucose) and glycogen synthase (UDP-glucose + glycogen[n] → UDP + glycogen[n+1]). Glucose-1-phosphate is found in virtually all organisms from bacteria to higher plants and animals. Measurement of intracellular G1P levels is crucial for analyzing the carbohydrate metabolic pathways and their kinetic properties

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    G1P Assay Buffer WM 1 x 27ml
    G1P Developer Green 1 vial
    G1P Enzyme Mix Purple 1 vial
    G1P Standard Yellow 1 vial
    G1P Substrate Mix Red 1 vial
  • Research areas

Images

  • Measurement of glucose-1-phosphate in jackfruit seed, bovine liver & jurkat cell lysate.
  • Typical Standard Curve

Protocols

References

ab155892 has not yet been referenced specifically in any publications.

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