Overview

  • Product name
    Glucose-6-Phosphate Assay Kit (Colorimetric)
    See all Glucose 6 phosphate kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type
    Quantitative
  • Sensitivity
    10 µM
  • Range
    1 µM - 30 µM
  • Assay time
    0h 40m
  • Product overview

    Glucose-6-Phosphate Assay Kit (Colorimetric) (ab83426) is a simple, sensitive and rapid test to quantify glucose-6-phospate (G6P) in a variety of samples. In the assay, glucose-6-phosphate is oxidized into an intermediate product which in turn converts the probe into an intensely colored product with an absorbance at OD=450 nm. This product can detect G6P in the range of 1-30 nmoles with a detection sensitivity ~10 µM G6P.

    For higher sensitivity, we recommend our fluorometric assay Glucose-6-Phosphate Assay Kit - High Sensitivity (Fluorometric) (ab107923).
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Glucose-6-phosphate (G6P) is a key sugar intermediate for glucose to get into cells, and then enter either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is transformed by G6PDH to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia.

  • Platform
    Microplate

Properties

Associated products

Images

  • Colorimetric standard curve: mean of duplicates (+/- SD) with background reads subtracted.

  • G6P (A) levels are elevated in GAA−/− mice compared to wild type (C57/Bl6) and reduced by rhGAA treatment.

    GAA−/− mice were dosed with rhGAA. G6P was quantified in heart and triceps homogenates from wild type (C57Bl/6) and GAA−/− mice using ab83426. Values are means ± SEM. Data was analyzed by one-way ANOVA followed by Newman-Keuls comparing groups. ***P<0.001.

Protocols

References

This product has been referenced in:
  • Pursell N  et al. Inhibition of Glycogen Synthase II with RNAi Prevents Liver Injury in Mouse Models of Glycogen Storage Diseases. Mol Ther N/A:N/A (2018). Functional Studies . Read more (PubMed: 29784585) »
  • Wu X  et al. IAPP modulates cellular autophagy, apoptosis, and extracellular matrix metabolism in human intervertebral disc cells. Cell Death Discov 3:16107 (2017). Read more (PubMed: 28149534) »
See all 5 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

Yes, you are correct that serum samples can be used directly. You can run a sample background control (omit Glucose 6 Phosphate Enzyme Mix from the reaction mix) with your serum samples and subtract the background OD from the sample readings.

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Question
Answer

Thank you for your inquiry.

I've heard back from the lab that this kit is specific for glucose 6 phosphate.



I hope this information helps. Please contact us with any other questions.

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Answer

Thank you for contacting us with your question.

To prepare the samples, we would recommend homogenizing the cells with a Dounce homogenizer, 10-50 passes while samples are on ice. Efficient lysis can be confirmed by viewing the cells under a microscope.

I hope this information will be useful, but please let me know if you have any further questions or if there is anything else that we can do for you.

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Answer

Please find below the answer from the lab :
5 % detergent will affect the enzyme assay and is NOT recommended.
I hope this is helpful.

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Answer


In order to give you an accurate answer to your question, could you please provide more details about the "high concentration of detergent"? What type of detergent and what concentration?

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Answer

Vielen Dank für Ihren Anruf vongestern. Ich habe unterdessen herausgefunden was passiert ist. In der Tat war das erste Substrat fehlerhaft. Wir hatten daher dieses Substrat in den betroffenen Kits ersetzt - mit dem neuen G6P substrate mix ab104231. Leider wurde dies fälschlicherweise einfach in das Kit getan, anstatt die Röhrchen umzutauschen. Der dunkelrote Substrat Mix (ab104231) ist der korrekte Mix. Wir werden Ihnen daher das erste Kit ersetzen - gleich mit einer neuen Lot Nummer. In Bezug auf das zweite Kit, können Sie wählen wie Sie vorgehen möchten: 1.) Sie benutzen das Kit wie vorgesehen, und benutzten nur den Mix ab104231. Falls dies doch nicht gute Resultate ergibt, werden wir es Ihnen auch ersetzen. 2.) Sie können uns das Kit zurücksenden, und wir senden Ihnen ein Neues oder geben Ihnen eine Gutschrift. Bitte lassen Sie mich auch wissen, ob Sie das Ersatzkit akzeptieren möchten und ob ich es an die gleiche Adresse wie die erste Bestellung senden kann. Könnten Sie uns auch die Rohdaten des Versuches mit dem zweiten Substrat Mix (ab104231) senden? Dies hätte nämlich gute Resultate geben sollen. Ich würde es daher gerne auch etwas genauer anschauen. Ich freue mich auf Ihre Antwort.

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Answer

Thank you for contacting us about the G6P assay kit today. I am waiting for the lab to reply regarding the perchloric acid de-proteinization protocol. Can you describe your samples to me? This step will be necessary for any cell lysate or tissue homogenate preparation. As an alternative, you may want to consider the 10kDa spin column, ab93349. We are currently unable to provide the perchloric acid kit, ab93299.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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