Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 20 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 1 µM
Product nameGlucose-6-Phosphate Assay Kit - High Sensitivity (Fluorometric)
See all Glucose 6 phosphate kits
Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
Sensitivity> 1 µM
Range1 µM - 500 µM
Assay time0h 20m
Abcam's Glucose-6-Phosphate Assay Kit - High Sensitivity (Fluorometric) (ab107923) is a simple, sensitive and rapid means of quantifying glucose-6-Phosphate (G6P) in a variety of samples. In the assay, glucose-6-phosphate is oxidized with the generation of a product that converts a nearly colorless probe to an intensely fluorescent product (Ex/Em 535/587 nm). This assay can detect G6P in the range of 10 to 500 pmoles which is equivalent to the range of 1-500 µM in the original sample assuming a dilution of 5X during processing. This Glucose-6-Phosphate Assay Kit - High Sensitivity is more sensitive than our colorimetric assay, Glucose-6-Phosphate Assay Kit (ab83426).
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Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells which then enters either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is utilized by its dehydrogenase to generate reducing equivalents in the form of NAD(P)H. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at -20°C. Please refer to protocols.
Storage bufferPreservative: None
Components Identifier 100 tests Glucose 6 phosphate assay buffer WM 1 x 25ml Glucose 6 phosphate Enzyme Mix (lyophilized) Green 1 vial Glucose 6 phosphate Standard (10 µmol; lyophilized) Yellow 1 vial Glucose 6 phosphate Substrate Mix (lyophilized) Red 1 vial Probe (in DMSO) Blue 1 x 0.4ml
RelevanceGlucose 6 phosphate (G6P) is a key sugar intermediate for glucose to get into cells, and then enter either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is utilized by its dehydrogenase to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia.
Glucose 6 phosphate measured in mouse tissue lysates showing quantity (pmol) per mg protein of tested sample
Glucose 6 phosphate measured in biological fluids showing quantity (pmol) per microliter of tested sample
Glucose 6 phosphate standard curves (0 – 500 and 0 – 50 pmol range) generated using ab107923.
ab107923 has been referenced in 1 publication.
- Carmean CM et al. Glycogen Repletion in Brown Adipose Tissue upon Refeeding Is Primarily Driven by Phosphorylation-Independent Mechanisms. PLoS One 11:e0156148 (2016). PubMed: 27213961